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1ybk

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[[Image:1ybk.gif|left|200px]]<br /><applet load="1ybk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ybk, resolution 1.45&Aring;" />
 
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'''RHCC cocrystallized with CAPB'''<br />
 
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==Overview==
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==RHCC cocrystallized with CAPB==
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Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A.
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<StructureSection load='1ybk' size='340' side='right'caption='[[1ybk]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ybk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YBK FirstGlance]. <br>
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1YBK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BEQ:'>BEQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM+INNER+SALT'>BEQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ybk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ybk OCA], [https://pdbe.org/1ybk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ybk RCSB], [https://www.ebi.ac.uk/pdbsum/1ybk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ybk ProSAT]</span></td></tr>
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Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805603 15805603]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q54436_STAMA Q54436_STAMA]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylothermus marinus]]
[[Category: Staphylothermus marinus]]
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[[Category: Stetefeld, J.]]
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[[Category: Stetefeld J]]
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[[Category: BEQ]]
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[[Category: SO4]]
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[[Category: right hand coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:03:41 2008''
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Current revision

RHCC cocrystallized with CAPB

PDB ID 1ybk

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