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1yn4

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[[Image:1yn4.gif|left|200px]]<br /><applet load="1yn4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yn4, resolution 1.80&Aring;" />
 
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'''Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens'''<br />
 
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==Overview==
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==Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens==
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The Eap (extracellular adherence protein) of Staphylococcus aureus functions as a secreted virulence factor by mediating interactions between the bacterial cell surface and several extracellular host proteins. Eap proteins from different Staphylococcal strains consist of four to six tandem repeats of a structurally uncharacterized domain (EAP domain). We have determined the three-dimensional structures of three different EAP domains to 1.8, 2.2, and 1.35 A resolution, respectively. These structures reveal a core fold that is comprised of an alpha-helix lying diagonally across a five-stranded, mixed beta-sheet. Comparison of EAP domains with known structures reveals an unexpected homology with the C-terminal domain of bacterial superantigens. Examination of the structure of the superantigen SEC2 bound to the beta-chain of a T-cell receptor suggests a possible ligand-binding site within the EAP domain (Fields, B. A., Malchiodi, E. L., Li, H., Ysern, X., Stauffacher, C. V., Schlievert, P. M., Karjalainen, K., and Mariuzza, R. (1996) Nature 384, 188-192). These results provide the first structural characterization of EAP domains, relate EAP domains to a large class of bacterial toxins, and will guide the design of future experiments to analyze EAP domain structure/function relationships.
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<StructureSection load='1yn4' size='340' side='right'caption='[[1yn4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yn4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YN4 FirstGlance]. <br>
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1YN4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YN4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yn4 OCA], [https://pdbe.org/1yn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yn4 RCSB], [https://www.ebi.ac.uk/pdbsum/1yn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yn4 ProSAT]</span></td></tr>
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The crystal structures of EAP domains from Staphylococcus aureus reveal an unexpected homology to bacterial superantigens., Geisbrecht BV, Hamaoka BY, Perman B, Zemla A, Leahy DJ, J Biol Chem. 2005 Apr 29;280(17):17243-50. Epub 2005 Feb 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15691839 15691839]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H3K0M1_STAAM A0A0H3K0M1_STAAM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1yn4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yn4 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Geisbrecht, B V.]]
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[[Category: Geisbrecht BV]]
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[[Category: Hamaoka, B Y.]]
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[[Category: Hamaoka BY]]
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[[Category: Leahy, D J.]]
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[[Category: Leahy DJ]]
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[[Category: Perman, B.]]
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[[Category: Perman B]]
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[[Category: Zemla, A.]]
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[[Category: Zemla A]]
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[[Category: ZN]]
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[[Category: extracellular adherence protein]]
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[[Category: staphylococcus aureus]]
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[[Category: toxin]]
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[[Category: virulence factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:01 2008''
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Current revision

Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens

PDB ID 1yn4

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