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1z0c

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[[Image:1z0c.gif|left|200px]]<br /><applet load="1z0c" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z0c, resolution 1.55&Aring;" />
 
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'''Crystal Structure of A. fulgidus Lon proteolytic domain D508A mutant'''<br />
 
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==Overview==
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==Crystal Structure of A. fulgidus Lon proteolytic domain D508A mutant==
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The atomic-resolution crystal structure of the proteolytic domain (P-domain, residues 415-621) of Archaeoglobus fulgidus B-type Lon protease (wtAfLonB) and the structures of several mutants have revealed significant differences in the conformation of the active-site residues when compared to other known Lon P-domains, despite the conservation of the overall fold. The catalytic Ser509 is facing the solvent and is distant from Lys552, the other member of the catalytic dyad. Instead, the adjacent Asp508 forms an ion pair with the catalytic lysine residue. Glu506, an analog of the putative third catalytic residue from a related Methanococcus jannaschii LonB, also faces the solvent and does not interact with the catalytic dyad. We have established that full-length wtAfLonB is proteolytically active in an ATP-dependent manner. The loss of enzymatic activity of the S509A mutant confirms the functional significance of this residue, while retention of considerable level of activity by the D508A and E506A mutants rules out their critical involvement in catalysis. In contrast to the full-length enzymes, all individually purified P-domains (wild-type and mutants) were inactive, and the mutations had no influence on the active-site structure. These findings raise the possibility that, although isolated proteolytic domains of both AfLonB and E.coli LonA are able to assemble into expected functional hexamers, the presence of the other domains, as well as substrate binding, may be needed to stabilize the productive conformation of their active sites. Thus, the observed conformational variability may reflect the differences in the stability of active-site structures for the proteolytic counterparts of single-chain Lon versus independently folded proteolytic subunits of two-chain AAA+ proteases.
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<StructureSection load='1z0c' size='340' side='right'caption='[[1z0c]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1z0c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z0C FirstGlance]. <br>
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1Z0C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/Endopeptidase_La Endopeptidase La], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.53 3.4.21.53] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0C OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z0c OCA], [https://pdbe.org/1z0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z0c RCSB], [https://www.ebi.ac.uk/pdbsum/1z0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z0c ProSAT]</span></td></tr>
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==Reference==
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</table>
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Atomic-resolution crystal structure of the proteolytic domain of Archaeoglobus fulgidus lon reveals the conformational variability in the active sites of lon proteases., Botos I, Melnikov EE, Cherry S, Kozlov S, Makhovskaya OV, Tropea JE, Gustchina A, Rotanova TV, Wlodawer A, J Mol Biol. 2005 Aug 5;351(1):144-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16002085 16002085]
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== Function ==
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[https://www.uniprot.org/uniprot/LONB_ARCFU LONB_ARCFU] ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/1z0c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z0c ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Endopeptidase La]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Botos I]]
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[[Category: Botos, I.]]
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[[Category: Cherry S]]
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[[Category: Cherry, S.]]
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[[Category: Gustchina A]]
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[[Category: Gustchina, A.]]
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[[Category: Kozlov S]]
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[[Category: Kozlov, S.]]
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[[Category: Makhovskaya OV]]
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[[Category: Makhovskaya, O V.]]
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[[Category: Melnikov EE]]
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[[Category: Melnikov, E E.]]
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[[Category: Rotanova TV]]
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[[Category: Rotanova, T V.]]
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[[Category: Tropea JE]]
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[[Category: Tropea, J E.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: atp-dependent protease]]
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[[Category: b-type lon]]
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[[Category: catalytic ser-lys dyad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:55 2008''
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Current revision

Crystal Structure of A. fulgidus Lon proteolytic domain D508A mutant

PDB ID 1z0c

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