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1z0f

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(New page: 200px<br /> <applet load="1z0f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z0f, resolution 2.15&Aring;" /> '''GDP-Bound Rab14 GTP...)
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[[Image:1z0f.gif|left|200px]]<br />
 
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<applet load="1z0f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z0f, resolution 2.15&Aring;" />
 
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'''GDP-Bound Rab14 GTPase'''<br />
 
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==Overview==
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==GDP-Bound Rab14 GTPase==
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Rab GTPases regulate all stages of membrane trafficking, including vesicle, budding, cargo sorting, transport, tethering and fusion. In the inactive, (GDP-bound) conformation, accessory factors facilitate the targeting of, Rab GTPases to intracellular compartments. After nucleotide exchange to, the active (GTP-bound) conformation, Rab GTPases interact with, functionally diverse effectors including lipid kinases, motor proteins and, tethering complexes. How effectors distinguish between homologous Rab, GTPases represents an unresolved problem with respect to the specificity, of vesicular trafficking. Using a structural proteomic approach, we have, determined the specificity and structural basis underlying the interaction, of the multivalent effector rabenosyn-5 with the Rab family. The results, demonstrate that even the structurally similar effector domains in, rabenosyn-5 can achieve highly selective recognition of distinct subsets, of Rab GTPases exclusively through interactions with the switch and, interswitch regions. The observed specificity is determined at a, family-wide level by structural diversity in the active conformation, which governs the spatial disposition of critical conserved recognition, determinants, and by a small number of both positive and negative sequence, determinants that allow further discrimination between Rab GTPases with, similar switch conformations.
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<StructureSection load='1z0f' size='340' side='right'caption='[[1z0f]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z0f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z0F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z0f OCA], [https://pdbe.org/1z0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z0f RCSB], [https://www.ebi.ac.uk/pdbsum/1z0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z0f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAB14_HUMAN RAB14_HUMAN] Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes (By similarity). Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion.<ref>PMID:22595670</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/1z0f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z0f ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Z0F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z0F OCA].
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*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural basis of family-wide Rab GTPase recognition by rabenosyn-5., Eathiraj S, Pan X, Ritacco C, Lambright DG, Nature. 2005 Jul 21;436(7049):415-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16034420 16034420]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Eathiraj, S.]]
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[[Category: Eathiraj S]]
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[[Category: Lambright, D.G.]]
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[[Category: Lambright DG]]
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[[Category: Pan, X.]]
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[[Category: Pan X]]
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[[Category: Ritacco, C.]]
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[[Category: Ritacco C]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: protein transport]]
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[[Category: rab gtpase]]
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[[Category: rab14]]
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[[Category: vesicular trafficking]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:27:58 2007''
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Current revision

GDP-Bound Rab14 GTPase

PDB ID 1z0f

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