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1z3i

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[[Image:1z3i.jpg|left|200px]]
 
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{{Structure
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==Structure of the SWI2/SNF2 chromatin remodeling domain of eukaryotic Rad54==
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|PDB= 1z3i |SIZE=350|CAPTION= <scene name='initialview01'>1z3i</scene>, resolution 3.00&Aring;
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<StructureSection load='1z3i' size='340' side='right'caption='[[1z3i]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1z3i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z3I FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3i OCA], [https://pdbe.org/1z3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z3i RCSB], [https://www.ebi.ac.uk/pdbsum/1z3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3i ProSAT]</span></td></tr>
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</table>
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'''Structure of the SWI2/SNF2 chromatin remodeling domain of eukaryotic Rad54'''
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== Function ==
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[https://www.uniprot.org/uniprot/RAD54_DANRE RAD54_DANRE] Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed:30961891). Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (By similarity).[UniProtKB:P32863]<ref>PMID:30961891</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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SWI2/SNF2 chromatin-remodeling proteins mediate the mobilization of nucleosomes and other DNA-associated proteins. SWI2/SNF2 proteins contain sequence motifs characteristic of SF2 helicases but do not have helicase activity. Instead, they couple ATP hydrolysis with the generation of superhelical torsion in DNA. The structure of the nucleosome-remodeling domain of zebrafish Rad54, a protein involved in Rad51-mediated homologous recombination, reveals that the core of the SWI2/SNF2 enzymes consist of two alpha/beta-lobes similar to SF2 helicases. The Rad54 helicase lobes contain insertions that form two helical domains, one within each lobe. These insertions contain SWI2/SNF2-specific sequence motifs likely to be central to SWI2/SNF2 function. A broad cleft formed by the two lobes and flanked by the helical insertions contains residues conserved in SWI2/SNF2 proteins and motifs implicated in DNA-binding by SF2 helicases. The Rad54 structure suggests that SWI2/SNF2 proteins use a mechanism analogous to helicases to translocate on dsDNA.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3i_consurf.spt"</scriptWhenChecked>
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1Z3I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3I OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the SWI2/SNF2 chromatin-remodeling domain of eukaryotic Rad54., Thoma NH, Czyzewski BK, Alexeev AA, Mazin AV, Kowalczykowski SC, Pavletich NP, Nat Struct Mol Biol. 2005 Apr;12(4):350-6. Epub 2005 Apr 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15806108 15806108]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z3i ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Danio rerio]]
[[Category: Danio rerio]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alexeev, A A.]]
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[[Category: Alexeev AA]]
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[[Category: Czyzewski, B K.]]
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[[Category: Czyzewski BK]]
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[[Category: Kowalczykowski, S C.]]
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[[Category: Kowalczykowski SC]]
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[[Category: Mazin, A V.]]
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[[Category: Mazin AV]]
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[[Category: Pavletich, N P.]]
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[[Category: Pavletich NP]]
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[[Category: Thoma, N H.]]
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[[Category: Thoma NH]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: recombination atpase helicase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:30:49 2008''
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Current revision

Structure of the SWI2/SNF2 chromatin remodeling domain of eukaryotic Rad54

PDB ID 1z3i

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