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1z90

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(New page: 200px<br /><applet load="1z90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z90, resolution 1.86&Aring;" /> '''X-ray structure of g...)
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[[Image:1z90.gif|left|200px]]<br /><applet load="1z90" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z90, resolution 1.86&Aring;" />
 
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'''X-ray structure of gene product from arabidopsis thaliana at3g03250, a putative UDP-glucose pyrophosphorylase'''<br />
 
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==Overview==
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==X-ray structure of gene product from arabidopsis thaliana at3g03250, a putative UDP-glucose pyrophosphorylase==
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The structure of the UDP-glucose pyrophosphorylase encoded by Arabidopsis, thaliana gene At3g03250 has been solved to a nominal resolution of 1.86 A., In addition, the structure has been solved in the presence of the, substrates/products UTP and UDP-glucose to nominal resolutions of 1.64 A, and 1.85 A. The three structures revealed a catalytic domain similar to, that of other nucleotidyl-glucose pyrophosphorylases with a, carboxy-terminal beta-helix domain in a unique orientation. Conformational, changes are observed between the native and substrate-bound complexes. The, nucleotide-binding loop and the carboxy-terminal domain, including the, suspected catalytically important Lys360, move in and out of the active, site in a concerted fashion. TLS refinement was employed initially to, model conformational heterogeneity in the UDP-glucose complex followed by, the use of multiconformer refinement for the entire molecule. Normal mode, analysis generated atomic displacement predictions in good agreement in, magnitude and direction with the observed conformational changes and, anisotropic displacement parameters generated by TLS refinement. The, structures and the observed dynamic changes provide insight into the, ordered mechanism of this enzyme and previously described oligomerization, effects on catalytic activity.
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<StructureSection load='1z90' size='340' side='right'caption='[[1z90]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1z90]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z90 FirstGlance]. <br>
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1Z90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z90 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z90 OCA], [https://pdbe.org/1z90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z90 RCSB], [https://www.ebi.ac.uk/pdbsum/1z90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z90 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure and Dynamics of UDP-Glucose Pyrophosphorylase from Arabidopsis thaliana with Bound UDP-Glucose and UTP., McCoy JG, Bitto E, Bingman CA, Wesenberg GE, Bannen RM, Kondrashov DA, Phillips GN Jr, J Mol Biol. 2007 Feb 23;366(3):830-41. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17178129 17178129]
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== Function ==
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[https://www.uniprot.org/uniprot/UGPA2_ARATH UGPA2_ARATH] Plays a central role as a glucosyl donor in cellular metabolic pathways (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z9/1z90_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z90 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Allard, S.T.M.]]
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[[Category: Allard STM]]
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[[Category: Bingman, C.A.]]
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[[Category: Bingman CA]]
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[[Category: Bitto, E.]]
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[[Category: Bitto E]]
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[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]
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[[Category: Phillips Jr GN]]
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[[Category: Jr., G.N.Phillips.]]
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[[Category: Wesenberg GE]]
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[[Category: Wesenberg, G.E.]]
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[[Category: at3g03250]]
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[[Category: center for eukaryotic structural genomics]]
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[[Category: cesg]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: putative udp-glucose pyrophosphorylase]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:18:39 2007''
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Current revision

X-ray structure of gene product from arabidopsis thaliana at3g03250, a putative UDP-glucose pyrophosphorylase

PDB ID 1z90

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