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1z96

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[[Image:1z96.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the Mud1 UBA domain==
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|PDB= 1z96 |SIZE=350|CAPTION= <scene name='initialview01'>1z96</scene>, resolution 1.80&Aring;
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<StructureSection load='1z96' size='340' side='right'caption='[[1z96]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1z96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z96 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= mud1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [https://pdbe.org/1z96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB], [https://www.ebi.ac.uk/pdbsum/1z96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z96 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal structure of the Mud1 UBA domain'''
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[https://www.uniprot.org/uniprot/MUD1_SCHPO MUD1_SCHPO]
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__TOC__
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</StructureSection>
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==Overview==
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[[Category: Large Structures]]
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The ubiquitin-pathway associated (UBA) domain is a 40-residue polyubiquitin-binding motif. The Schizosaccharomyces pombe protein Mud1 is an ortholog of the Saccharomyces cerevisiae DNA-damage response protein Ddi1 and binds to K48-linked polyubiquitin through its UBA domain. We have solved the crystal structure of Mud1 UBA at 1.8 angstroms resolution, revealing a canonical three-helical UBA fold. We have probed the interactions of this domain using mutagenesis, surface plasmon resonance, NMR and analytical ultracentrifugation. We show that the ubiquitin-binding surface of Mud1 UBA extends beyond previously recognized motifs and can be functionally dissected into primary and secondary ubiquitin-binding sites. Mutation of Phe330 to alanine, a residue exposed between helices 2 and 3, significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyubiquitin, despite leaving the primary binding surface functionally intact. Moreover, K48-linked diubiquitin binds a single Mud1 UBA domain even in the presence of excess UBA. We therefore propose a mechanism for the recognition of K48-linked polyubiquitin chains by Mud1 in which diubiquitin units are specifically recognized by a single UBA domain.
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==About this Structure==
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1Z96 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA].
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==Reference==
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Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16138082 16138082]
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Single protein]]
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[[Category: Brown NR]]
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[[Category: Brown, N R.]]
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[[Category: Campbell ID]]
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[[Category: Campbell, I D.]]
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[[Category: Endicott JA]]
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[[Category: Endicott, J A.]]
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[[Category: Gordon C]]
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[[Category: Gordon, C.]]
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[[Category: Johnson LN]]
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[[Category: Johnson, L N.]]
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[[Category: Lowe ED]]
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[[Category: Lowe, E D.]]
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[[Category: Noble MEM]]
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[[Category: Noble, M E.M.]]
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[[Category: Trempe J-F]]
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[[Category: Trempe, J F.]]
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[[Category: three-helix bundle]]
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[[Category: uba]]
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[[Category: ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:40 2008''
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Current revision

Crystal structure of the Mud1 UBA domain

PDB ID 1z96

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