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1zdu

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[[Image:1zdu.gif|left|200px]]
 
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{{Structure
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==The Crystal Structure of Human Liver Receptor Homologue-1==
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|PDB= 1zdu |SIZE=350|CAPTION= <scene name='initialview01'>1zdu</scene>, resolution 2.50&Aring;
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<StructureSection load='1zdu' size='340' side='right'caption='[[1zdu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=P3A:PHOSPHATIDYLGLYCEROL-PHOSPHOGLYCEROL'>P3A</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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<table><tr><td colspan='2'>[[1zdu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZDU FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P3A:PHOSPHATIDYLGLYCEROL-PHOSPHOGLYCEROL'>P3A</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zdu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zdu OCA], [https://pdbe.org/1zdu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zdu RCSB], [https://www.ebi.ac.uk/pdbsum/1zdu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zdu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NR5A2_HUMAN NR5A2_HUMAN] Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zd/1zdu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zdu ConSurf].
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<div style="clear:both"></div>
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'''The Crystal Structure of Human Liver Receptor Homologue-1'''
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==See Also==
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*[[Liver receptor homolog-1|Liver receptor homolog-1]]
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__TOC__
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==Overview==
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</StructureSection>
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Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for development of endodermal tissues and regulates cholesterol and bile acid homeostasis. Regulatory ligands are unknown for SF-1 and LRH-1. A reported mouse LRH-1 structure revealed an empty pocket in a region commonly occupied by ligands in the structures of other nuclear receptors, and pocket-filling mutations did not alter the constitutive activity observed. Here we report the crystal structures of the putative ligand-binding domains of human SF-1 at 2.1-A resolution and human LRH-1 at 2.5-A resolution. Both structures bind a coactivator-derived peptide at the canonical activation-function surface, thus adopting the transcriptionally activating conformation. In human LRH-1, coactivator peptide binding also occurs to a second site. We discovered in both structures a phospholipid molecule bound in a pocket of the putative ligand-binding domain. MS analysis of the protein samples used for crystallization indicated that the two proteins associate with a range of phospholipids. Mutations of the pocket-lining residues reduced the transcriptional activities of SF-1 and LRH-1 in mammalian cell transfection assays without affecting their expression levels. These results suggest that human SF-1 and LRH-1 may be ligand-binding receptors, although it remains to be seen if phospholipids or possibly other molecules regulate SF-1 or LRH-1 under physiological conditions.
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==About this Structure==
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1ZDU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDU OCA].
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==Reference==
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The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1., Wang W, Zhang C, Marimuthu A, Krupka HI, Tabrizizad M, Shelloe R, Mehra U, Eng K, Nguyen H, Settachatgul C, Powell B, Milburn MV, West BL, Proc Natl Acad Sci U S A. 2005 May 24;102(21):7505-10. Epub 2005 May 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15897460 15897460]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Eng, K.]]
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[[Category: Eng K]]
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[[Category: Krupka, H I.]]
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[[Category: Krupka HI]]
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[[Category: Marimuthu, A.]]
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[[Category: Marimuthu A]]
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[[Category: Mehra, U.]]
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[[Category: Mehra U]]
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[[Category: Milburn, M V.]]
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[[Category: Milburn MV]]
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[[Category: Nguyen, H.]]
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[[Category: Nguyen H]]
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[[Category: Powell, B.]]
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[[Category: Powell B]]
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[[Category: Settachatgul, C.]]
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[[Category: Settachatgul C]]
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[[Category: Shelloe, R.]]
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[[Category: Shelloe R]]
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[[Category: Tabrizizad, M.]]
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[[Category: Tabrizizad M]]
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[[Category: Wang, W.]]
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[[Category: Wang W]]
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[[Category: West, B L.]]
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[[Category: West BL]]
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[[Category: Zhang, C.]]
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[[Category: Zhang C]]
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[[Category: P3A]]
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[[Category: TRS]]
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[[Category: liver receptor homologue-1]]
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[[Category: lrh-1]]
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[[Category: nuclear receptor]]
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[[Category: phosphatidylethanolamine]]
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[[Category: phospholipid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:34:17 2008''
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Current revision

The Crystal Structure of Human Liver Receptor Homologue-1

PDB ID 1zdu

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