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1zdw

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[[Image:1zdw.gif|left|200px]]<br /><applet load="1zdw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zdw, resolution 2.02&Aring;" />
 
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'''Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GSPP and Flaviolin'''<br />
 
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==Overview==
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==Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GSPP and Flaviolin==
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The anti-oxidant naphterpin is a natural product containing a polyketide-based aromatic core with an attached 10-carbon geranyl group derived from isoprenoid (terpene) metabolism. Hybrid natural products such as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl) or 15-carbon (farnesyl) isoprenoid chains possess biological activities distinct from their non-prenylated aromatic precursors. These hybrid natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of aromatic prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated and characterized. Here we report the gene identification, biochemical characterization and high-resolution X-ray crystal structures of an architecturally novel aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and carbon-oxygen-based prenylation of a diverse collection of hydroxyl-containing aromatic acceptors of synthetic, microbial and plant origin. These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases.
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<StructureSection load='1zdw' size='340' side='right'caption='[[1zdw]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zdw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._CL190 Streptomyces sp. CL190]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZDW FirstGlance]. <br>
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1ZDW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=FLV:'>FLV</scene> and <scene name='pdbligand=GST:'>GST</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLV:FLAVIOLIN'>FLV</scene>, <scene name='pdbligand=GST:GERANYL+S-THIOLODIPHOSPHATE'>GST</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zdw OCA], [https://pdbe.org/1zdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zdw RCSB], [https://www.ebi.ac.uk/pdbsum/1zdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zdw ProSAT]</span></td></tr>
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Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products., Kuzuyama T, Noel JP, Richard SB, Nature. 2005 Jun 16;435(7044):983-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15959519 15959519]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Streptomyces sp.]]
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[https://www.uniprot.org/uniprot/Q4R2T2_STRC1 Q4R2T2_STRC1]
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[[Category: Kuzuyama, T.]]
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== Evolutionary Conservation ==
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[[Category: Noel, J P.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Richard, S B.]]
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Check<jmol>
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[[Category: FLV]]
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<jmolCheckbox>
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[[Category: GST]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zd/1zdw_consurf.spt"</scriptWhenChecked>
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[[Category: MG]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: NO3]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: novel aromatic prenyltransferase barrel fold]]
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</jmolCheckbox>
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[[Category: pt-barrel]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zdw ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:14:39 2008''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces sp. CL190]]
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[[Category: Kuzuyama T]]
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[[Category: Noel JP]]
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[[Category: Richard SB]]

Current revision

Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GSPP and Flaviolin

PDB ID 1zdw

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