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1zo3

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(New page: 200px<br /><applet load="1zo3" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zo3" /> '''The P-site and P/E-site tRNA structures fitt...)
Current revision (09:06, 14 February 2024) (edit) (undo)
 
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[[Image:1zo3.gif|left|200px]]<br /><applet load="1zo3" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zo3" />
 
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'''The P-site and P/E-site tRNA structures fitted to P/I site codon.'''<br />
 
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==Overview==
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==The P-site and P/E-site tRNA structures fitted to P/I site codon.==
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The 70S ribosome and its complement of factors required for initiation of, translation in E. coli were purified separately and reassembled in vitro, with GDPNP, producing a stable initiation complex (IC) stalled after 70S, assembly. We have obtained a cryo-EM reconstruction of the IC showing, IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP, contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here, adopts a conformation radically different from that seen in the recent, crystal structure of IF2. The C-terminal domain of IF2 binds to the, single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into, a novel orientation at the P site. The GTP binding domain of IF2 binds to, the GTPase-associated center of the 50S subunit in a manner similar to, EF-G and EF-Tu. Additionally, we present evidence for the localization of, IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of, IF2 in the initiation complex.
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<SX load='1zo3' size='340' side='right' viewer='molstar' caption='[[1zo3]], [[Resolution|resolution]] 13.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZO3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo3 OCA], [https://pdbe.org/1zo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zo3 RCSB], [https://www.ebi.ac.uk/pdbsum/1zo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zo3 ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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1ZO3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA].
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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__TOC__
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==Reference==
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</SX>
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The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15935757 15935757]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Allen, G.S.]]
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[[Category: Allen GS]]
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[[Category: Ehrenberg, M.]]
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[[Category: Ehrenberg M]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Gursky, R.]]
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[[Category: Gursky R]]
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[[Category: Zavialov, A.]]
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[[Category: Zavialov A]]
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[[Category: cryo-eletron microscopy]]
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[[Category: e. coli]]
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[[Category: initiation factor]]
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[[Category: initiation of protein synthesis]]
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[[Category: ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:39:09 2008''
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Current revision

The P-site and P/E-site tRNA structures fitted to P/I site codon.

1zo3, resolution 13.80Å

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