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1zo3
From Proteopedia
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==The P-site and P/E-site tRNA structures fitted to P/I site codon.== | ==The P-site and P/E-site tRNA structures fitted to P/I site codon.== | ||
| - | < | + | <SX load='1zo3' size='340' side='right' viewer='molstar' caption='[[1zo3]], [[Resolution|resolution]] 13.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZO3 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo3 OCA], [https://pdbe.org/1zo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zo3 RCSB], [https://www.ebi.ac.uk/pdbsum/1zo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zo3 ProSAT]</span></td></tr> |
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex. | ||
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| - | The cryo-EM structure of a translation initiation complex from Escherichia coli.,Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J Cell. 2005 Jun 3;121(5):703-12. PMID:15935757<ref>PMID:15935757</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | |
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__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Allen | + | [[Category: Large Structures]] |
| - | [[Category: Ehrenberg | + | [[Category: Allen GS]] |
| - | [[Category: Frank | + | [[Category: Ehrenberg M]] |
| - | [[Category: Gursky | + | [[Category: Frank J]] |
| - | [[Category: Zavialov | + | [[Category: Gursky R]] |
| - | + | [[Category: Zavialov A]] | |
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Current revision
The P-site and P/E-site tRNA structures fitted to P/I site codon.
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