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1zww

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[[Image:1zww.gif|left|200px]]<br /><applet load="1zww" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zww, resolution 2.30&Aring;" />
 
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'''Crystal structure of endophilin-A1 BAR domain'''<br />
 
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==Overview==
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==Crystal structure of endophilin-A1 BAR domain==
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Endophilin has been implicated in the retrieval of membrane via endocytosis of clathrin-coated vesicles, which is crucial for the maintenance of neurotransmitter exocytosis during stimulation; both exocytosis and endocytosis are regulated by intracellular calcium levels. Here, we present the 2.3 A crystal structure of the endophilin-A1 BAR domain, which has been suggested to function in inducing and sensing membrane curvature at the site of endocytosis. Endo-BAR folds into a crescent-shaped dimer composed of two elongated, three-helix bundles. Two additional domains of 30 residues each, inserted into helix 1 at the center of the concave side of the dimer, may interfere with the proposed mode of BAR domain membrane interaction. In addition, the dimer binds 11 divalent cadmium ions in the crystal mostly with typical Ca2+ co-ordination spheres. The endophilin-1A BAR domain thus constitutes a new variant of a BAR domain, and it may link endophilin-1A BAR function to calcium regulation of endocytosis.
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<StructureSection load='1zww' size='340' side='right'caption='[[1zww]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zww]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZWW FirstGlance]. <br>
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1ZWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zww OCA], [https://pdbe.org/1zww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zww RCSB], [https://www.ebi.ac.uk/pdbsum/1zww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zww ProSAT]</span></td></tr>
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Crystal structure of the endophilin-A1 BAR domain., Weissenhorn W, J Mol Biol. 2005 Aug 19;351(3):653-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16023669 16023669]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SH3G2_MOUSE SH3G2_MOUSE] Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.<ref>PMID:10490020</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/1zww_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zww ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Weissenhorn W]]
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[[Category: Weissenhorn, W.]]
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[[Category: CD]]
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[[Category: coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:19:52 2008''
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Current revision

Crystal structure of endophilin-A1 BAR domain

PDB ID 1zww

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