1zy5

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[[Image:1zy5.gif|left|200px]]
 
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==Crystal Structure of eIF2alpha Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with AMPPNP.==
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The line below this paragraph, containing "STRUCTURE_1zy5", creates the "Structure Box" on the page.
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<StructureSection load='1zy5' size='340' side='right'caption='[[1zy5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zy5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZY5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1zy5| PDB=1zy5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zy5 OCA], [https://pdbe.org/1zy5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zy5 RCSB], [https://www.ebi.ac.uk/pdbsum/1zy5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zy5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCN2_YEAST GCN2_YEAST] Stimulates GCN4 translation in amino acid-starved cells by phosphorylating the alpha subunit of eIF-2 (SUI2) on 'Ser-52'.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zy5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zy5 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of eIF2alpha Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with AMPPNP.'''
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==See Also==
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*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The GCN2 protein kinase coordinates protein synthesis with levels of amino acid stores by phosphorylating eukaryotic translation initiation factor 2. The autoinhibited form of GCN2 is activated in cells starved of amino acids by binding of uncharged tRNA to a histidyl-tRNA synthetase-like domain. Replacement of Arg-794 with Gly in the PK domain (R794G) activates GCN2 independently of tRNA binding. Crystal structures of the GCN2 protein kinase domain have been determined for wild-type and R794G mutant forms in the apo state and bound to ATP/AMPPNP. These structures reveal that GCN2 autoinhibition results from stabilization of a closed conformation that restricts ATP binding. The R794G mutant shows increased flexibility in the hinge region connecting the N- and C-lobes, resulting from loss of multiple interactions involving Arg794. This conformational change is associated with intradomain movement that enhances ATP binding and hydrolysis. We propose that intramolecular interactions following tRNA binding remodel the hinge region in a manner similar to the mechanism of enzyme activation elicited by the R794G mutation.
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[[Category: Large Structures]]
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==About this Structure==
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1ZY5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY5 OCA].
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==Reference==
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Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2., Padyana AK, Qiu H, Roll-Mecak A, Hinnebusch AG, Burley SK, J Biol Chem. 2005 Aug 12;280(32):29289-99. Epub 2005 Jun 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15964839 15964839]
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Burley SK]]
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[[Category: Burley, S K.]]
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[[Category: Hinnebusch AG]]
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[[Category: Hinnebusch, A G.]]
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[[Category: Padyana AK]]
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[[Category: Padyana, A K.]]
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[[Category: Qiu H]]
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[[Category: Qiu, H.]]
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[[Category: Roll-Mecak A]]
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[[Category: Roll-Mecak, A.]]
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[[Category: Amino-acid starvation]]
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[[Category: Eif2alpha kinase]]
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[[Category: Protein kinase]]
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[[Category: Signal transduction]]
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[[Category: Starvation stress response]]
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[[Category: Translation regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:13:30 2008''
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Current revision

Crystal Structure of eIF2alpha Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with AMPPNP.

PDB ID 1zy5

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