2a0b

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[[Image:2a0b.gif|left|200px]]<br />
 
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<applet load="2a0b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2a0b, resolution 1.57&Aring;" />
 
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'''HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI'''<br />
 
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==Overview==
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==HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI==
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The crystal structure of the histidine-containing phosphotransfer (HPt), domain of the anaerobic sensor kinase ArcB from Escherichia coli has been, refined to 1.57 A resolution, using the coordinates of the earlier 2.06 A, structure as a starting model. The final model contained 956 protein, atoms, one zinc ion and 156 water molecules, with an R factor of 19.0%., The high-resolution electron-density maps clearly revealed additional, solvent molecules and seven discrete rotamers in the protein side chains., One residue, Met755, was fully buried but was able to occupy the space in, the hydrophobic core by means of the two-state conformation of its side, chain. One water molecule was buried in the protein core and contributed, to the rigidity of the HPt domain, cooperating in the coordination of the, zinc ion.
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<StructureSection load='2a0b' size='340' side='right'caption='[[2a0b]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2a0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A0B FirstGlance]. <br>
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2A0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Sites: PHB and ZNB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A0B OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a0b OCA], [https://pdbe.org/2a0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a0b RCSB], [https://www.ebi.ac.uk/pdbsum/2a0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a0b ProSAT]</span></td></tr>
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Refined structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57 A resolution., Kato M, Mizuno T, Shimizu T, Hakoshima T, Acta Crystallogr D Biol Crystallogr. 1999 Nov;55(Pt 11):1842-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10531481 10531481]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARCB_ECOLI ARCB_ECOLI] Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/2a0b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a0b ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hakoshima, T.]]
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[[Category: Hakoshima T]]
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[[Category: Kato, M.]]
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[[Category: Kato M]]
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[[Category: Mizuno, T.]]
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[[Category: Mizuno T]]
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[[Category: Shimizu, T.]]
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[[Category: Shimizu T]]
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[[Category: ZN]]
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[[Category: four-helix bundle]]
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[[Category: histidine kinase]]
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[[Category: phosphotransfer]]
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[[Category: sensory transduction]]
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[[Category: two-component system]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:34:44 2007''
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Current revision

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

PDB ID 2a0b

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