2a2f

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[[Image:2a2f.gif|left|200px]]<br /><applet load="2a2f" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2a2f, resolution 2.50&Aring;" />
 
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'''Crystal Structure of Sec15 C-terminal domain'''<br />
 
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==Overview==
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==Crystal Structure of Sec15 C-terminal domain==
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Sec15, a component of the exocyst, recognizes vesicle-associated Rab GTPases, helps target transport vesicles to the budding sites in yeast and is thought to recruit other exocyst proteins. Here we report the characterization of a 35-kDa fragment that comprises most of the C-terminal half of Drosophila melanogaster Sec15. This C-terminal domain was found to bind a subset of Rab GTPases, especially Rab11, in a GTP-dependent manner. We also provide evidence that in fly photoreceptors Sec15 colocalizes with Rab11 and that loss of Sec15 affects rhabdomere morphology. Determination of the 2.5-A crystal structure of the C-terminal domain revealed a novel fold consisting of ten alpha-helices equally distributed between two subdomains (N and C subdomains). We show that the C subdomain, mainly via a single helix, is sufficient for Rab binding.
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<StructureSection load='2a2f' size='340' side='right'caption='[[2a2f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2a2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A2F FirstGlance]. <br>
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2A2F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2F OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a2f OCA], [https://pdbe.org/2a2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a2f RCSB], [https://www.ebi.ac.uk/pdbsum/2a2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a2f ProSAT]</span></td></tr>
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==Reference==
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</table>
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Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo., Wu S, Mehta SQ, Pichaud F, Bellen HJ, Quiocho FA, Nat Struct Mol Biol. 2005 Oct;12(10):879-85. Epub 2005 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16155582 16155582]
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== Function ==
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[https://www.uniprot.org/uniprot/EXOC6_DROME EXOC6_DROME] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/2a2f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a2f ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bellen, H J.]]
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[[Category: Bellen HJ]]
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[[Category: Mehta, S Q.]]
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[[Category: Mehta SQ]]
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[[Category: Pichaud, F.]]
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[[Category: Pichaud F]]
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[[Category: Quiocho, F A.]]
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[[Category: Quiocho FA]]
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[[Category: Wu, S.]]
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[[Category: Wu S]]
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[[Category: all helical structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:55 2008''
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Current revision

Crystal Structure of Sec15 C-terminal domain

PDB ID 2a2f

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