2a3v

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[[Image:2a3v.gif|left|200px]]
 
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{{Structure
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==Structural basis for broad DNA-specificity in integron recombination==
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|PDB= 2a3v |SIZE=350|CAPTION= <scene name='initialview01'>2a3v</scene>, resolution 2.8&Aring;
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<StructureSection load='2a3v' size='340' side='right'caption='[[2a3v]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2a3v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3V FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE= intI4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 Vibrio cholerae O1 biovar eltor str. N16961])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3v OCA], [https://pdbe.org/2a3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3v RCSB], [https://www.ebi.ac.uk/pdbsum/2a3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3v ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O68847_VIBCL O68847_VIBCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3v ConSurf].
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<div style="clear:both"></div>
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'''Structural basis for broad DNA-specificity in integron recombination'''
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==See Also==
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Lateral DNA transfer--the movement of genetic traits between bacteria--has a profound impact on genomic evolution and speciation. The efficiency with which bacteria incorporate genetic information reflects their capacity to adapt to changing environmental conditions. Integron integrases are proteins that mediate site-specific DNA recombination between a proximal primary site (attI) and a secondary target site (attC) found within mobile gene cassettes encoding resistance or virulence factors. The lack of sequence conservation among attC sites has led to the hypothesis that a sequence-independent structural recognition determinant must exist within attC. Here we report the crystal structure of an integron integrase bound to an attC substrate. The structure shows that DNA target site recognition and high-order synaptic assembly are not dependent on canonical DNA but on the position of two flipped-out bases that interact in cis and in trans with the integrase. These extrahelical bases, one of which is required for recombination in vivo, originate from folding of the bottom strand of attC owing to its imperfect internal dyad symmetry. The mechanism reported here supports a new paradigm for how sequence-degenerate single-stranded genetic material is recognized and exchanged between bacteria.
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[[Category: Large Structures]]
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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==About this Structure==
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[[Category: Bouvier M]]
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2A3V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_eltor_str._n16961 Vibrio cholerae o1 biovar eltor str. n16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3V OCA].
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[[Category: Demarre G]]
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[[Category: Gopaul DN]]
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==Reference==
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[[Category: MacDonald D]]
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Structural basis for broad DNA-specificity in integron recombination., MacDonald D, Demarre G, Bouvier M, Mazel D, Gopaul DN, Nature. 2006 Apr 27;440(7088):1157-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16641988 16641988]
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[[Category: Mazel D]]
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[[Category: Single protein]]
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[[Category: Vibrio cholerae o1 biovar eltor str. n16961]]
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[[Category: Bouvier, M.]]
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[[Category: Demarre, G.]]
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[[Category: Gopaul, D N.]]
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[[Category: MacDonald, D.]]
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[[Category: Mazel, D.]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:03 2008''
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Current revision

Structural basis for broad DNA-specificity in integron recombination

PDB ID 2a3v

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