2abh

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(New page: 200px<br /><applet load="2abh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2abh, resolution 1.7&Aring;" /> '''PHOSPHATE-BINDING PRO...)
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[[Image:2abh.gif|left|200px]]<br /><applet load="2abh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2abh, resolution 1.7&Aring;" />
 
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'''PHOSPHATE-BINDING PROTEIN (RE-REFINED)'''<br />
 
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==Overview==
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==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==
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Electrostatic interactions are among the key forces determining the, structure and function of proteins. These are exemplified in the liganded, form of the receptor, a phosphate binding protein from Escherichia coli., The phosphate, completely dehydrated and buried in the receptor, is bound, by 12 hydrogen bonds as well as a salt link with Arg 135. We have, modulated the ionic attraction while preserving the hydrogen bonds by, mutating Asp 137, also salt linked to Arg 135, to Asn, Gly or Thr., High-resolution crystallographic analysis revealed that Gly and Thr (but, not Asn) mutant proteins have incorporated a more electronegative Cl- in, place of the Asp carboxylate. That no dramatic effect on phosphate, affinity was produced by these ionic perturbations indicates a major role, for hydrogen bonds and other local dipoles in the binding and charge, stabilization of ionic ligands.
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<StructureSection load='2abh' size='340' side='right'caption='[[2abh]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [https://pdbe.org/2abh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [https://www.ebi.ac.uk/pdbsum/2abh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI] Part of the ABC transporter complex PstSACB involved in phosphate import.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/2abh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2abh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2ABH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1ABH. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA].
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*[[Phosphate-binding protein|Phosphate-binding protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Modulation of a salt link does not affect binding of phosphate to its specific active transport receptor., Yao N, Ledvina PS, Choudhary A, Quiocho FA, Biochemistry. 1996 Feb 20;35(7):2079-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8652549 8652549]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choudhary, A.]]
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[[Category: Choudhary A]]
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[[Category: Ledvina, P.S.]]
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[[Category: Ledvina PS]]
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[[Category: Quiocho, F.A.]]
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[[Category: Quiocho FA]]
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[[Category: Yao, N.]]
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[[Category: Yao N]]
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[[Category: PO4]]
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[[Category: phosphotransferase]]
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[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:02:11 2007''
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Current revision

PHOSPHATE-BINDING PROTEIN (RE-REFINED)

PDB ID 2abh

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