2au1

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[[Image:2au1.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of group A Streptococcus MAC-1 orthorhombic form==
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|PDB= 2au1 |SIZE=350|CAPTION= <scene name='initialview01'>2au1</scene>, resolution 2.4&Aring;
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<StructureSection load='2au1' size='340' side='right'caption='[[2au1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>
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<table><tr><td colspan='2'>[[2au1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS5005 Streptococcus pyogenes MGAS5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AU1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2au1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2au1 OCA], [https://pdbe.org/2au1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2au1 RCSB], [https://www.ebi.ac.uk/pdbsum/2au1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2au1 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2au1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2au1 OCA], [http://www.ebi.ac.uk/pdbsum/2au1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2au1 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q7DAM2_STRP1 Q7DAM2_STRP1]
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== Evolutionary Conservation ==
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'''Crystal Structure of group A Streptococcus MAC-1 orthorhombic form'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/2au1_consurf.spt"</scriptWhenChecked>
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Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2AU1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas5005 Streptococcus pyogenes mgas5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AU1 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2au1 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472742 16472742]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Streptococcus pyogenes mgas5005]]
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[[Category: Streptococcus pyogenes MGAS5005]]
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[[Category: Agniswamy, J.]]
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[[Category: Agniswamy J]]
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[[Category: Liu, M.]]
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[[Category: Liu M]]
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[[Category: Musser, J M.]]
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[[Category: Musser JM]]
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[[Category: Nagiec, M J.]]
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[[Category: Nagiec MJ]]
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[[Category: Schuck, P.]]
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[[Category: Schuck P]]
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[[Category: Sun, P D.]]
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[[Category: Sun PD]]
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[[Category: mac-1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:16 2008''
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Current revision

Crystal Structure of group A Streptococcus MAC-1 orthorhombic form

PDB ID 2au1

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