2avu

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(New page: 200px<br /><applet load="2avu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2avu, resolution 3.00&Aring;" /> '''Structure of the Esc...)
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[[Image:2avu.gif|left|200px]]<br /><applet load="2avu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2avu, resolution 3.00&Aring;" />
 
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'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription'''<br />
 
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==Overview==
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==Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription==
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The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC), regulates transcription from several flagellar and non-flagellar operons, in bacteria. The crystallographic structure of the Escherichia coli FlhDC, complex has been solved to 3.0 A resolution, revealing a hexameric, FlhD4FlhC2 assembly. In the complex, each FlhC protomer binds an FlhD2, dimer; the conformation of the dimer in the complex differs significantly, from its conformation in the absence of FlhC. FlhC has a novel tertiary, fold that includes a heretofore unrecognized zinc-binding site in which, the ion is ligated by four cysteine residues. Gel shift experiments show, that binding of the FlhDC complex to a cognate promoter bends the DNA by, approximately 111 degrees . The structure of the FlhDC complex is, compatible with models in which a fragment of operator DNA, at least 48, base-pairs in length, wraps around the complex and bends significantly, when binding.
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<StructureSection load='2avu' size='340' side='right'caption='[[2avu]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2avu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AVU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2avu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avu OCA], [https://pdbe.org/2avu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2avu RCSB], [https://www.ebi.ac.uk/pdbsum/2avu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2avu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLHD_ECOLI FLHD_ECOLI] Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.<ref>PMID:7961507</ref> <ref>PMID:11169100</ref> <ref>PMID:15941987</ref> <ref>PMID:18765794</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/2avu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2avu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2AVU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AVU OCA].
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16337229 16337229]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Fleming, R.T.]]
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[[Category: Fleming RT]]
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[[Category: Matsumura, P.]]
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[[Category: Matsumura P]]
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[[Category: McKay, D.B.]]
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[[Category: McKay DB]]
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[[Category: Wang, S.]]
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[[Category: Wang S]]
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[[Category: Westbrook, E.M.]]
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[[Category: Westbrook EM]]
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[[Category: ZN]]
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[[Category: c4-type zinc finger]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:23:44 2007''
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Current revision

Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription

PDB ID 2avu

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