This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2b5a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2b5a" size="350" color="white" frame="true" align="right" spinBox="true" caption="2b5a, resolution 1.543&Aring;" /> '''C.BclI, Control Ele...)
Current revision (09:16, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2b5a.gif|left|200px]]<br /><applet load="2b5a" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2b5a, resolution 1.543&Aring;" />
 
-
'''C.BclI, Control Element of the BclI Restriction-Modification System'''<br />
 
-
==Overview==
+
==C.BclI, Control Element of the BclI Restriction-Modification System==
-
Protection from DNA invasion is afforded by restriction-modification, systems in many bacteria. The efficiency of protection depends crucially, on the relative expression levels of restriction versus methytransferase, genes. This regulation is provided by a controller protein, named C, protein. Studies of the Bcll system in E. coli suggest that C.Bcll, functions as a negative regulator for M.Bcll expression, implying that it, plays a role in defense against foreign DNA during virus infection. C.Bcll, binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that, overlaps with the putative -35 promoter region upstream of the bcllM and, bcllC genes. The C.Bcll fold comprises five alpha helices: two helices, form a helix-turn-helix motif, and the remaining three helices form the, extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA, bending might play an important role in gene regulation, and that Glu27, and Asp31 in C.Bcll might function critically in the regulation.
+
<StructureSection load='2b5a' size='340' side='right'caption='[[2b5a]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2b5a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5A FirstGlance]. <br>
-
2B5A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus] with <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.543&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [https://pdbe.org/2b5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB], [https://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5a ProSAT]</span></td></tr>
-
Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16338412 16338412]
+
</table>
-
[[Category: Bacillus caldolyticus]]
+
== Evolutionary Conservation ==
-
[[Category: Protein complex]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Balendiran, G.K.]]
+
Check<jmol>
-
[[Category: Chan, S.H.]]
+
<jmolCheckbox>
-
[[Category: Dabur, R.]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5a_consurf.spt"</scriptWhenChecked>
-
[[Category: Mersha, F.]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: Sawaya, M.R.]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: Xu, S.Y.]]
+
</jmolCheckbox>
-
[[Category: Zhu, Z.]]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b5a ConSurf].
-
[[Category: ACY]]
+
<div style="clear:both"></div>
-
[[Category: helix-turn-helix motif]]
+
__TOC__
-
 
+
</StructureSection>
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:17:09 2008''
+
[[Category: Large Structures]]
 +
[[Category: Balendiran GK]]
 +
[[Category: Chan SH]]
 +
[[Category: Dabur R]]
 +
[[Category: Mersha F]]
 +
[[Category: Sawaya MR]]
 +
[[Category: Xu SY]]
 +
[[Category: Zhu Z]]

Current revision

C.BclI, Control Element of the BclI Restriction-Modification System

PDB ID 2b5a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b5a"
Personal tools