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2bpa

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(New page: 200px<br /><applet load="2bpa" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bpa, resolution 3.000&Aring;" /> '''ATOMIC STRUCTURE OF...)
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[[Image:2bpa.gif|left|200px]]<br /><applet load="2bpa" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bpa, resolution 3.000&Aring;" />
 
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'''ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS'''<br />
 
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==Overview==
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==ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS==
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The mechanism of DNA ejection, viral assembly and evolution are related to, the structure of bacteriophage phi X174. The F protein forms a T = 1, capsid whose major folding motif is the eight-stranded antiparallel beta, barrel found in many other icosahedral viruses. Groups of 5 G proteins, form 12 dominating spikes that enclose a hydrophilic channel containing, some diffuse electron density. Each G protein is a tight beta barrel with, its strands running radially outwards and with a topology similar to that, of the F protein. The 12 'pilot' H proteins per virion may be partially, located in the putative ion channel. The small, basic J protein is, associated with the DNA and is situated in an interior cleft of the F, protein. Tentatively, there are three regions of partially ordered DNA, structure,
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<StructureSection load='2bpa' size='340' side='right'caption='[[2bpa]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bpa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_phiX174 Escherichia virus phiX174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BPA FirstGlance]. <br>
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2BPA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_phix174 Enterobacteria phage phix174]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BPA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bpa OCA], [https://pdbe.org/2bpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bpa RCSB], [https://www.ebi.ac.uk/pdbsum/2bpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bpa ProSAT]</span></td></tr>
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==Reference==
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</table>
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Atomic structure of single-stranded DNA bacteriophage phi X174 and its functional implications., McKenna R, Xia D, Willingmann P, Ilag LL, Krishnaswamy S, Rossmann MG, Olson NH, Baker TS, Incardona NL, Nature. 1992 Jan 9;355(6356):137-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1370343 1370343]
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== Function ==
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[[Category: Enterobacteria phage phix174]]
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[https://www.uniprot.org/uniprot/CAPSD_BPPHS CAPSD_BPPHS] Assembles to form an icosahedral capsid with a T=1 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins F (PubMed:11991963, PubMed:1370343, PubMed:8158636). Upon virus binding to host cell, one of the spikes dissociates from the capsid and the virus interacts with LPS through the exposed EF loops on the F proteins (PubMed:29229840). After the genome had been ejected, the channel formed by the F proteins at the unique fivefold axis remains open (PubMed:29229840).<ref>PMID:11991963</ref> <ref>PMID:1370343</ref> <ref>PMID:29229840</ref> <ref>PMID:8158636</ref>
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[[Category: Protein complex]]
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== Evolutionary Conservation ==
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[[Category: Baker, T.S.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ilag, L.L.]]
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Check<jmol>
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[[Category: Incardona, N.L.]]
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<jmolCheckbox>
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[[Category: Krishnaswamy, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bp/2bpa_consurf.spt"</scriptWhenChecked>
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[[Category: McKenna, R.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Olson, N.H.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Rossmann, M.G.]]
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</jmolCheckbox>
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[[Category: Willingmann, P.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bpa ConSurf].
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[[Category: Xia, D.]]
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<div style="clear:both"></div>
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[[Category: icosahedral virus]]
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== References ==
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[[Category: protein-dna complex]]
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<references/>
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[[Category: single strand]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:51:54 2007''
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[[Category: Escherichia virus phiX174]]
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[[Category: Large Structures]]
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[[Category: Baker TS]]
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[[Category: Ilag LL]]
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[[Category: Incardona NL]]
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[[Category: Krishnaswamy S]]
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[[Category: McKenna R]]
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[[Category: Olson NH]]
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[[Category: Rossmann MG]]
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[[Category: Willingmann P]]
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[[Category: Xia D]]

Current revision

ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS

PDB ID 2bpa

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