2drp

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(New page: 200px<br /><applet load="2drp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2drp, resolution 2.800&Aring;" /> '''THE CRYSTAL STRUCTU...)
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[[Image:2drp.gif|left|200px]]<br /><applet load="2drp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2drp, resolution 2.800&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF A TWO ZINC-FINGER PEPTIDE REVEALS AN EXTENSION TO THE RULES FOR ZINC-FINGER/DNA RECOGNITION'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF A TWO ZINC-FINGER PEPTIDE REVEALS AN EXTENSION TO THE RULES FOR ZINC-FINGER/DNA RECOGNITION==
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The Cys2-His2 zinc-finger is the most widely occurring DNA-binding motif., The first structure of a zinc-finger/DNA complex revealed a fairly simple, mechanism for DNA recognition suggesting that the zinc-finger might, represent a candidate template for designing proteins to recognize DNA., Residues at three key positions in an alpha-helical 'reading head' play a, dominant role in base-recognition and have been targets for mutagenesis, experiments aimed at deriving a recognition code. Here we report the, structure of a two zinc-finger DNA-binding domain from the protein, Tramtrack complexed with DNA. The amino-terminal zinc-finger and its, interaction with DNA illustrate several novel features. These include the, use of a serine residue, which is semi-conserved and located outside the, three key positions, to make a base contact. Its role in base-recognition, correlates with a large, local, protein-induced deformation of the DNA, helix at a flexible A-T-A sequence and may give insight into previous, mutagenesis experiments. It is apparent from this structure that, zinc-finger/DNA recognition is more complex than was originally perceived.
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<StructureSection load='2drp' size='340' side='right'caption='[[2drp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2drp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DRP FirstGlance]. <br>
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2DRP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DRP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2drp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2drp OCA], [https://pdbe.org/2drp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2drp RCSB], [https://www.ebi.ac.uk/pdbsum/2drp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2drp ProSAT]</span></td></tr>
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The crystal structure of a two zinc-finger peptide reveals an extension to the rules for zinc-finger/DNA recognition., Fairall L, Schwabe JW, Chapman L, Finch JT, Rhodes D, Nature. 1993 Dec 2;366(6454):483-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8247159 8247159]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TTKB_DROME TTKB_DROME] Binds to a number of sites in the transcriptional regulatory region of ftz. Isoform beta is required to repress inappropriate segmentation gene transcription and repress genes incompatible with development of photoreceptor cell fates. Probable repressor of the transcription of the segmentation genes ftz, eve, h, odd, run, and en. Inhibits Trl-dependent activation of eve. May bind to the region AGGGC/TGG. Degradation of ttk is directed by binding of sinah or sina, via the adapter molecule phyl which binds to the BTB domain of ttk. A second method of degradation exists that is phyl-independent, this is mediated by recognition of motifs in the C-terminus of ttk.<ref>PMID:2104801</ref> <ref>PMID:8223261</ref> <ref>PMID:8504931</ref> <ref>PMID:12384587</ref> <ref>PMID:17962185</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dr/2drp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2drp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chapman, L.]]
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[[Category: Chapman L]]
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[[Category: Fairall, L.]]
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[[Category: Fairall L]]
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[[Category: Finch, J.T.]]
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[[Category: Finch JT]]
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[[Category: Rhodes, D.]]
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[[Category: Rhodes D]]
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[[Category: Schwabe, J.W.R.]]
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[[Category: Schwabe JWR]]
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[[Category: ZN]]
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[[Category: double helix]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:44:54 2007''
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Current revision

THE CRYSTAL STRUCTURE OF A TWO ZINC-FINGER PEPTIDE REVEALS AN EXTENSION TO THE RULES FOR ZINC-FINGER/DNA RECOGNITION

PDB ID 2drp

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