2f23

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[[Image:2f23.gif|left|200px]]
 
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==Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus==
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The line below this paragraph, containing "STRUCTURE_2f23", creates the "Structure Box" on the page.
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<StructureSection load='2f23' size='340' side='right'caption='[[2f23]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f23]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F23 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f23 OCA], [https://pdbe.org/2f23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f23 RCSB], [https://www.ebi.ac.uk/pdbsum/2f23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f23 ProSAT]</span></td></tr>
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{{STRUCTURE_2f23| PDB=2f23 | SCENE= }}
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</table>
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== Function ==
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'''Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus'''
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[https://www.uniprot.org/uniprot/GFH1_THET2 GFH1_THET2] Inhibits all catalytic activities of RNA polymerase (RNAP) by partially occluding its substrate-binding site and preventing NTP binding.<ref>PMID:16628221</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Gfh1, a transcription factor from Thermus thermophilus, inhibits all catalytic activities of RNA polymerase (RNAP). We characterized the Gfh1 structure, function and possible mechanism of action and regulation. Gfh1 inhibits RNAP by competing with NTPs for coordinating the active site Mg2+ ion. This coordination requires at least two aspartates at the tip of the Gfh1 N-terminal coiled-coil domain (NTD). The overall structure of Gfh1 is similar to that of the Escherichia coli transcript cleavage factor GreA, except for the flipped orientation of the C-terminal domain (CTD). We show that depending on pH, Gfh1-CTD exists in two alternative orientations. At pH above 7, it assumes an inactive 'flipped' orientation seen in the structure, which prevents Gfh1 from binding to RNAP. At lower pH, Gfh1-CTD switches to an active 'Gre-like' orientation, which enables Gfh1 to bind to and inhibit RNAP.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/2f23_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2F23 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F23 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f23 ConSurf].
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pH-dependent conformational switch activates the inhibitor of transcription elongation., Laptenko O, Kim SS, Lee J, Starodubtseva M, Cava F, Berenguer J, Kong XP, Borukhov S, EMBO J. 2006 May 17;25(10):2131-41. Epub 2006 Apr 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16628221 16628221]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Thermus thermophilus]]
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<references/>
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[[Category: Kim, S S.]]
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__TOC__
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[[Category: Kong, X P.]]
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</StructureSection>
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[[Category: Crystal structure anti-grea gfh1 thermus thermophilus]]
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:22:20 2008''
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[[Category: Thermus thermophilus HB27]]
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[[Category: Kim S-S]]
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[[Category: Kong XP]]

Current revision

Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus

PDB ID 2f23

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