2f6m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:21, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2f6m.gif|left|200px]]<br /><applet load="2f6m" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2f6m, resolution 2.10&Aring;" />
 
-
'''Structure of a Vps23-C:Vps28-N subcomplex'''<br />
 
-
==Overview==
+
==Structure of a Vps23-C:Vps28-N subcomplex==
-
The endosomal sorting complex required for transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 A resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners.
+
<StructureSection load='2f6m' size='340' side='right'caption='[[2f6m]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2f6m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F6M FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f6m OCA], [https://pdbe.org/2f6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f6m RCSB], [https://www.ebi.ac.uk/pdbsum/2f6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f6m ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/STP22_YEAST STP22_YEAST] Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1.<ref>PMID:10207082</ref> <ref>PMID:11208108</ref> <ref>PMID:11511343</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/2f6m_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f6m ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2F6M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=DDQ:'>DDQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F6M OCA].
+
*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Structural and functional organization of the ESCRT-I trafficking complex., Kostelansky MS, Sun J, Lee S, Kim J, Ghirlando R, Hierro A, Emr SD, Hurley JH, Cell. 2006 Apr 7;125(1):113-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16615894 16615894]
+
__TOC__
-
[[Category: Protein complex]]
+
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Hurley, J H.]]
+
[[Category: Hurley JH]]
-
[[Category: Kim, J.]]
+
[[Category: Kim J]]
-
[[Category: Kostelansky, M S.]]
+
[[Category: Kostelansky MS]]
-
[[Category: Lee, S.]]
+
[[Category: Lee S]]
-
[[Category: DDQ]]
+
-
[[Category: MG]]
+
-
[[Category: endosomal sorting complex required for transport]]
+
-
[[Category: endosomes]]
+
-
[[Category: escrt protein complexes]]
+
-
[[Category: escrt-i]]
+
-
[[Category: trafficking complex]]
+
-
[[Category: tsg101]]
+
-
[[Category: ubiquitin]]
+
-
[[Category: vacuole protein sorting]]
+
-
[[Category: vps23]]
+
-
[[Category: vps28]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:15 2008''
+

Current revision

Structure of a Vps23-C:Vps28-N subcomplex

PDB ID 2f6m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f6m"
Personal tools