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2fb3

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(New page: 200px<br /><applet load="2fb3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fb3, resolution 2.349&Aring;" /> '''Structure of MoaA i...)
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[[Image:2fb3.gif|left|200px]]<br /><applet load="2fb3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fb3, resolution 2.349&Aring;" />
 
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'''Structure of MoaA in complex with 5'-GTP'''<br />
 
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==Overview==
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==Structure of MoaA in complex with 5'-GTP==
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The first step in molybdenum cofactor biosynthesis, the conversion of, 5'-GTP to precursor Z, an oxygen-sensitive tetrahydropyranopterin is, catalyzed by the S-adenosylmethionine (SAM)-dependent enzyme MoaA and the, accessory protein MoaC. This reaction involves the radical-initiated, intramolecular rearrangement of the guanine C8 atom. MoaA harbors an, N-terminal [4Fe-4S] cluster, which is involved in the reductive cleavage, of SAM and generates a 5'-deoxyadenosyl radical (5'-dA*), and a C-terminal, [4Fe-4S] cluster presumably involved in substrate binding and/or, activation. Biochemical studies identified residues involved in 5'-GTP, binding and the determinants of nucleotide specificity. The crystal, structure of MoaA in complex with 5'-GTP confirms the biochemical data and, provides valuable insights into the subsequent radical reaction. MoaA, binds 5'-GTP with high affinity and interacts through its C-terminal, [4Fe-4S] cluster with the guanine N1 and N2 atoms, in a yet, uncharacterized binding mode. The tightly anchored triphosphate moiety, prevents the escape of radical intermediates. This structure also, visualizes the L-Met and 5'-dA cleavage products of SAM. Rotation of the, 5'-dA ribose and/or conformational changes of the guanosine are proposed, to bring the 5'-deoxyadenosyl radical into close proximity of either the, ribose C2' and C3' or the guanine C8 carbon atoms leading to hydrogen, abstraction.
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<StructureSection load='2fb3' size='340' side='right'caption='[[2fb3]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fb3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FB3 FirstGlance]. <br>
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2FB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with SO4, MET, SF4, GTP, 5AD and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FB3 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.349&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fb3 OCA], [https://pdbe.org/2fb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fb3 RCSB], [https://www.ebi.ac.uk/pdbsum/2fb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fb3 ProSAT]</span></td></tr>
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Binding of 5'-GTP to the C-terminal FeS cluster of the radical S-adenosylmethionine enzyme MoaA provides insights into its mechanism., Hanzelmann P, Schindelin H, Proc Natl Acad Sci U S A. 2006 May 2;103(18):6829-34. Epub 2006 Apr 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16632608 16632608]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/MOAA_STAA8 MOAA_STAA8] Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).<ref>PMID:15317939</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fb3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fb3 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Haenzelmann, P.]]
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[[Category: Haenzelmann P]]
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[[Category: Schindelin, H.]]
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[[Category: Schindelin H]]
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[[Category: 5AD]]
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[[Category: GTP]]
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[[Category: MET]]
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[[Category: POP]]
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[[Category: SF4]]
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[[Category: SO4]]
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[[Category: 5'-deoxyadenosine]]
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[[Category: 5'-gtp]]
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[[Category: [4fe-4s] clusters]]
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[[Category: s-adenosylmethionine]]
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[[Category: tim barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:27:52 2007''
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Current revision

Structure of MoaA in complex with 5'-GTP

PDB ID 2fb3

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