This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2fez

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:23, 14 February 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2fez.gif|left|200px]]
 
-
<!--
+
==Mycobacterium tuberculosis EmbR==
-
The line below this paragraph, containing "STRUCTURE_2fez", creates the "Structure Box" on the page.
+
<StructureSection load='2fez' size='340' side='right'caption='[[2fez]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2fez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FEZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fez OCA], [https://pdbe.org/2fez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fez RCSB], [https://www.ebi.ac.uk/pdbsum/2fez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fez ProSAT]</span></td></tr>
-
{{STRUCTURE_2fez| PDB=2fez | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''Mycobacterium tuberculosis EmbR'''
+
[https://www.uniprot.org/uniprot/EMBR_MYCTU EMBR_MYCTU] Positively regulates the transcription of the embCAB operon. Exhibits ATPase and GTPase activities.<ref>PMID:16817899</ref> <ref>PMID:16585755</ref>
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
Ser/Thr phosphorylation has emerged as a critical regulatory mechanism in a number of bacteria, including Mycobacterium tuberculosis. This problematic pathogen encodes 11 eukaryotic-like Ser/Thr kinases, yet few substrates or signaling targets have been characterized. Here, we report the structure of EmbR (2.0 A), a putative transcriptional regulator of key arabinosyltransferases (EmbC, -A, and -B), and an endogenous substrate of the Ser/Thr-kinase PknH. EmbR presents a unique domain architecture: the N-terminal winged-helix DNA-binding domain forms an extensive interface with the all-helical central bacterial transcriptional activation domain and is positioned adjacent to the regulatory C-terminal forkhead-associated (FHA) domain, which mediates binding to a Thr-phosphorylated site in PknH. The structure in complex with a phospho-peptide (1.9 A) reveals a conserved mode of phospho-threonine recognition by the FHA domain and evidence for specific recognition of the cognate kinase. The present structures suggest hypotheses as to how EmbR might propagate the phospho-relay signal from its cognate kinase, while serving as a template for the structurally uncharacterized Streptomyces antibiotic regulatory protein family of transcription factors.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/2fez_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
2FEZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEZ OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fez ConSurf].
-
Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis., Alderwick LJ, Molle V, Kremer L, Cozzone AJ, Dafforn TR, Besra GS, Futterer K, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2558-63. Epub 2006 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16477027 16477027]
+
<div style="clear:both"></div>
-
[[Category: Mycobacterium tuberculosis]]
+
== References ==
-
[[Category: Single protein]]
+
<references/>
-
[[Category: Alderwick, L J.]]
+
__TOC__
-
[[Category: Besra, G S.]]
+
</StructureSection>
-
[[Category: Futterer, K.]]
+
[[Category: Large Structures]]
-
[[Category: Beta-sandwich;]]
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
[[Category: Tetratricopeptide repeat]]
+
[[Category: Alderwick LJ]]
-
[[Category: Transcriptional regulator]]
+
[[Category: Besra GS]]
-
[[Category: Winged-helix]]
+
[[Category: Futterer K]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:49:04 2008''
+

Current revision

Mycobacterium tuberculosis EmbR

PDB ID 2fez

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fez"
Personal tools