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2fkg

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[[Image:2fkg.gif|left|200px]]<br /><applet load="2fkg" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fkg, resolution 2.40&Aring;" />
 
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'''The Crystal Structure of Engineered OspA'''<br />
 
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==Overview==
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==The Crystal Structure of Engineered OspA==
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Although the beta-rich self-assemblies are a major structural class for, polypeptides and the focus of intense research, little is known about, their atomic structures and dynamics due to their insoluble and, noncrystalline nature. We developed a protein engineering strategy that, captures a self-assembly segment in a water-soluble molecule. A predefined, number of self-assembling peptide units are linked, and the beta-sheet, ends are capped to prevent aggregation, which yields a mono-dispersed, soluble protein. We tested this strategy by using Borrelia outer surface, protein (OspA) whose single-layer beta-sheet located between two globular, domains consists of two beta-hairpin units and thus can be considered as a, prototype of self-assembly. We constructed self-assembly mimics of, different sizes and determined their atomic structures using x-ray, crystallography and NMR spectroscopy. Highly regular beta-sheet geometries, were maintained in these structures, and peptide units had a nearly, identical conformation, supporting the concept that a peptide in the, regular beta-geometry is primed for self-assembly. However, we found small, but significant differences in the relative orientation between adjacent, peptide units in terms of beta-sheet twist and bend, suggesting their, inherent flexibility. Modeling shows how this conformational diversity, when propagated over a large number of peptide units, can lead to a, substantial degree of nanoscale polymorphism of self-assemblies.
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<StructureSection load='2fkg' size='340' side='right'caption='[[2fkg]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fkg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FKG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fkg OCA], [https://pdbe.org/2fkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fkg RCSB], [https://www.ebi.ac.uk/pdbsum/2fkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fkg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OSPA_BORBU OSPA_BORBU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/2fkg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fkg ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FKG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FKG OCA].
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*[[Outer surface protein|Outer surface protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Atomic structures of peptide self-assembly mimics., Makabe K, McElheny D, Tereshko V, Hilyard A, Gawlak G, Yan S, Koide A, Koide S, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17753-8. Epub 2006 Nov 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17093048 17093048]
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[[Category: Borreliella burgdorferi]]
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[[Category: Borrelia burgdorferi]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Gawlak G]]
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[[Category: Gawlak, G.]]
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[[Category: Koide S]]
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[[Category: Koide, S.]]
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[[Category: Makabe K]]
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[[Category: Makabe, K.]]
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[[Category: Terechko V]]
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[[Category: Terechko, V.]]
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[[Category: Yan S]]
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[[Category: Yan, S.]]
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[[Category: beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:13 2008''
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Current revision

The Crystal Structure of Engineered OspA

PDB ID 2fkg

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