2fl7

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[[Image:2fl7.gif|left|200px]]
 
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==S. cerevisiae Sir3 BAH domain==
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The line below this paragraph, containing "STRUCTURE_2fl7", creates the "Structure Box" on the page.
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<StructureSection load='2fl7' size='340' side='right'caption='[[2fl7]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fl7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FL7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl7 OCA], [https://pdbe.org/2fl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fl7 RCSB], [https://www.ebi.ac.uk/pdbsum/2fl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fl7 ProSAT]</span></td></tr>
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{{STRUCTURE_2fl7| PDB=2fl7 | SCENE= }}
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</table>
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== Function ==
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'''S. cerevisiae Sir3 BAH domain'''
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[https://www.uniprot.org/uniprot/SIR3_YEAST SIR3_YEAST] The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure. Involves the compaction of chromatin fiber into a more condensed form.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Sir3p is a silent-information-regulator (SIR) protein required for the assembly of a transcriptionally "silent" chromatin structure at telomeres and the cryptic HM mating-type loci in Saccharomyces cerevisiae. Sir3p contains a putative "bromo adjacent homology" (BAH) domain at its N terminus that shares strong sequence similarity with the BAH domain of a subunit of the origin recognition complex (ORC), Orc1p. The Orc1p-BAH domain forms a well-defined complex with the ORC interaction region (OIR) of another Sir protein, Sir1p, which targets formation of silent chromatin to the HM-loci. Interestingly, despite sequence similarity of the Sir3p and Orc1p BAH domains and Sir3p's established importance in silencing, Sir3p does not bind the Sir1p-OIR. Here we report the 1.95 A resolution crystal structure of the Sir3p-BAH domain. The structure reveals two key features that can account for Sir3p-BAH domain's inability to interact with Sir1p. First, several Orc1p-BAH domain residues known to directly contact Sir1p are altered in the Sir3p-BAH domain. Second, a critical OIR-binding pocket present on the surface of the Orc1p-BAH domain is "filled" in the Sir3p-BAH domain structure, potentially making it inaccessible to Sir1p. These findings imply that the Sir3p-BAH domain structure has evolved for functions distinct from those of the Orc1p-BAH domain.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2fl7_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2FL7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL7 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fl7 ConSurf].
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Structure of the Sir3 protein bromo adjacent homology (BAH) domain from S. cerevisiae at 1.95 A resolution., Hou Z, Danzer JR, Fox CA, Keck JL, Protein Sci. 2006 May;15(5):1182-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16641491 16641491]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Daner JR]]
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[[Category: Daner, J R.]]
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[[Category: Fox CA]]
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[[Category: Fox, C A.]]
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[[Category: Hou Z]]
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[[Category: Hou, Z.]]
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[[Category: Keck JL]]
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[[Category: Keck, J L.]]
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[[Category: Chromatin]]
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[[Category: Orc]]
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[[Category: Silencing]]
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[[Category: Sir]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:01:21 2008''
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Current revision

S. cerevisiae Sir3 BAH domain

PDB ID 2fl7

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