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2fqn

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[[Image:2fqn.gif|left|200px]]<br /><applet load="2fqn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fqn, resolution 2.300&Aring;" />
 
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'''Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site'''<br />
 
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==Overview==
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==Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site==
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The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493.
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<StructureSection load='2fqn' size='340' side='right'caption='[[2fqn]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fqn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQN FirstGlance]. <br>
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2FQN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqn OCA], [https://pdbe.org/2fqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqn RCSB], [https://www.ebi.ac.uk/pdbsum/2fqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqn ProSAT]</span></td></tr>
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Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16452297 16452297]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Kondo, J.]]
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</StructureSection>
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[[Category: Urzhumtsev, A.]]
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[[Category: Large Structures]]
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[[Category: Westhof, E.]]
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[[Category: Kondo J]]
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[[Category: MG]]
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[[Category: Urzhumtsev A]]
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[[Category: NCO]]
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[[Category: Westhof E]]
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[[Category: a site]]
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[[Category: decoding site]]
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[[Category: homo sapiens cytoplasm]]
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[[Category: rna duplex]]
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[[Category: rrna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:09 2008''
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Current revision

Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site

PDB ID 2fqn

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