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2fw4

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[[Image:2fw4.gif|left|200px]]<br /><applet load="2fw4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fw4, resolution 2.00&Aring;" />
 
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'''Carbonic anhydrase activators. The first X-ray crystallographic study of an activator of isoform I, structure with L-histidine.'''<br />
 
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==Overview==
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==Carbonic anhydrase activators. The first X-ray crystallographic study of an activator of isoform I, structure with L-histidine.==
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The X-ray crystallographic structure for the adduct of an activator with, human carbonic anhydrase isozyme I (hCA I) is reported. L-Histidine binds, deep within the enzyme active site, participating in a network of hydrogen, bonds involving its carboxylate moiety and the zinc-bound water molecule, as well as the imidazole of His200, being in van der Waals contacts with, Thr199, His200, His64, and His67. This binding is very different from that, to the other major cytosolic isozyme hCA II.
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<StructureSection load='2fw4' size='340' side='right'caption='[[2fw4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fw4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FW4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fw4 OCA], [https://pdbe.org/2fw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fw4 RCSB], [https://www.ebi.ac.uk/pdbsum/2fw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fw4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAH1_HUMAN CAH1_HUMAN] Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.<ref>PMID:10550681</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/2fw4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fw4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FW4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=HIS:'>HIS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FW4 OCA].
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*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Carbonic anhydrase activators: the first X-ray crystallographic study of an adduct of isoform I., Temperini C, Scozzafava A, Supuran CT, Bioorg Med Chem Lett. 2006 Oct 1;16(19):5152-6. Epub 2006 Jul 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16870440 16870440]
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__TOC__
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[[Category: Carbonate dehydratase]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Scozzafava, A.]]
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[[Category: Scozzafava A]]
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[[Category: Supuran, C.T.]]
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[[Category: Supuran CT]]
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[[Category: Temperini, C.]]
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[[Category: Temperini C]]
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[[Category: HIS]]
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[[Category: ZN]]
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[[Category: activators]]
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[[Category: carbonic anhydrase i]]
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[[Category: crystal structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:26:23 2008''
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Current revision

Carbonic anhydrase activators. The first X-ray crystallographic study of an activator of isoform I, structure with L-histidine.

PDB ID 2fw4

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