This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2g0b

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:26, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2g0b.gif|left|200px]]
 
-
{{Structure
+
==The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes==
-
|PDB= 2g0b |SIZE=350|CAPTION= <scene name='initialview01'>2g0b</scene>, resolution 3.000&Aring;
+
<StructureSection load='2g0b' size='340' side='right'caption='[[2g0b]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=NLT:N-DODECANOYL-L-TYROSINE'>NLT</scene>
+
<table><tr><td colspan='2'>[[2g0b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G0B FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-
|GENE= feem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77133 uncultured bacterium])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NLT:N-DODECANOYL-L-TYROSINE'>NLT</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0b OCA], [https://pdbe.org/2g0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g0b RCSB], [https://www.ebi.ac.uk/pdbsum/2g0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g0b ProSAT]</span></td></tr>
-
 
+
</table>
-
'''The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/Q8KNZ7_9BACT Q8KNZ7_9BACT]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Attempts to access antibiotics by capturing biosynthetic genes and pathways directly from environmental DNA, which is overwhelmingly derived from uncultured bacteria, have revealed a large and previously unknown family of N-acyl amino acid synthases (NASs). The structure of the NAS FeeM reveals structural similarity to the GCN5-related N-acyl transferases and acylhomoserine lactone synthases. The overall structure has a central beta sheet with alpha helices on both sides. A bound product at a cleft in the beta sheet identifies the active site and the structural basis for catalysis, and sequence conservation in this region indicates a bias for recognition over speed. FeeM interacts with an acyl carrier protein (FeeL), and the structure, mutagenesis, and enzymatic measurements reveal that a small hydrophobic pocket in alpha helix 5 dominates binding of FeeM to FeeL. The structural and mechanistic analyses suggest that the products of FeeM could be bacterial signaling agents.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g0/2g0b_consurf.spt"</scriptWhenChecked>
-
2G0B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0B OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding., Van Wagoner RM, Clardy J, Structure. 2006 Sep;14(9):1425-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16962973 16962973]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g0b ConSurf].
-
[[Category: Single protein]]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Uncultured bacterium]]
[[Category: Uncultured bacterium]]
-
[[Category: Clardy, J.]]
+
[[Category: Clardy J]]
-
[[Category: Wagoner, R M.Van.]]
+
[[Category: Van Wagoner RM]]
-
[[Category: NLT]]
+
-
[[Category: antibiotic synthase]]
+
-
[[Category: environmental dna]]
+
-
[[Category: n-acyl transferase]]
+
-
[[Category: protein-product complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:53 2008''
+

Current revision

The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes

PDB ID 2g0b

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g0b"
Personal tools