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2g3h

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(New page: 200px<br /> <applet load="2g3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g3h, resolution 1.40&Aring;" /> '''Cyanide Binding and...)
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[[Image:2g3h.gif|left|200px]]<br />
 
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<applet load="2g3h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2g3h, resolution 1.40&Aring;" />
 
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'''Cyanide Binding and Heme Cavity Conformational Transitions in Drosophila melanogaster Hexa-coordinate Hemoglobin'''<br />
 
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==Overview==
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==Cyanide Binding and Heme Cavity Conformational Transitions in Drosophila melanogaster Hexa-coordinate Hemoglobin==
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The reason for the presence of hemoglobin-like molecules in insects, such, as Drosophila melanogaster, that live in fully aerobic environments has, yet to be determined. Heme endogenous hexacoordination (where HisE7 and, HisF8 axial ligands to the heme Fe atom are both provided by the protein), is a recently discovered mechanism proposed to modulate O(2) affinity in, hemoglobins from different species. Previous results have shown that D., melanogaster hemoglobin 1 (product of the glob1 gene) displays heme, endogenous hexacoordination in both the ferrous and ferric states. Here we, present kinetic data characterizing the exogenous cyanide ligand binding, process, and the three-dimensional structure (at 1.4 A resolution) of the, ensuing cyano-met D. melanogaster hemoglobin. Comparison with the crystal, structure of the endogenously hexacoordinated D. melanogaster hemoglobin, shows that the transition to the cyano-met form is supported by, conformational readjustment in the CD-D-E region of the protein, which, removes HisE7 from the heme. The structural and functional features of D., melanogaster hemoglobin are examined in light of previous results achieved, for human and mouse neuroglobins and for human cytoglobin, which display, heme endogenous hexacoordination. The study shows that, despite the rather, constant value for cyanide association rate constants for the ferric, hemoproteins, different distal site conformational readjustments and/or, heme sliding mechanisms are displayed by the known hexacoordinate, hemoglobins as a result of exogenous ligand binding.
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<StructureSection load='2g3h' size='340' side='right'caption='[[2g3h]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2g3h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G3H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3h OCA], [https://pdbe.org/2g3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g3h RCSB], [https://www.ebi.ac.uk/pdbsum/2g3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9VF15_DROME Q9VF15_DROME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/2g3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g3h ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2G3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with CL, MG, CYN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G3H OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Cyanide binding and heme cavity conformational transitions in Drosophila melanogaster hexacoordinate hemoglobin., de Sanctis D, Ascenzi P, Bocedi A, Dewilde S, Burmester T, Hankeln T, Moens L, Bolognesi M, Biochemistry. 2006 Aug 22;45(33):10054-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16906763 16906763]
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ascenzi, P.]]
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[[Category: Ascenzi P]]
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[[Category: Bocedi, A.]]
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[[Category: Bocedi A]]
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[[Category: Bolognesi, M.]]
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[[Category: Bolognesi M]]
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[[Category: Burmester, T.]]
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[[Category: Burmester T]]
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[[Category: Dewilde, S.]]
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[[Category: Dewilde S]]
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[[Category: Hankeln, T.]]
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[[Category: Hankeln T]]
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[[Category: Moens, L.]]
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[[Category: Moens L]]
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[[Category: Sanctis, D.de.]]
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[[Category: De Sanctis D]]
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[[Category: CL]]
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[[Category: CYN]]
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[[Category: HEM]]
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[[Category: MG]]
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[[Category: drosophila melanogaster hemoglobin structure; hexa-coordinate hemoglobin; cyanide binding to hemoglobin; heme distal site structure; fruit fly hemoglobin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:30:02 2007''
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Current revision

Cyanide Binding and Heme Cavity Conformational Transitions in Drosophila melanogaster Hexa-coordinate Hemoglobin

PDB ID 2g3h

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