2gis

[[Image:2gis.gif|left|200px]]

{{Structure

|PDB= 2gis |SIZE=350|CAPTION= <scene name='initialview01'>2gis</scene>, resolution 2.90&Aring;

|SITE=

|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>

|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gis OCA], [http://www.ebi.ac.uk/pdbsum/2gis PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gis RCSB]</span>

 

 

==Overview==

Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.

 

2GIS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA].

 

Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16810258 16810258]

[[Category: Protein complex]]

[[Category: Batey, R T.]]

[[Category: Montange, R K.]]

[[Category: mrna]]

[[Category: riboswitch]]