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2gjw

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(New page: 200px<br /><applet load="2gjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gjw, resolution 2.85&Aring;" /> '''RNA Recognition and ...)
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[[Image:2gjw.gif|left|200px]]<br /><applet load="2gjw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gjw, resolution 2.85&Aring;" />
 
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'''RNA Recognition and Cleavage by an Splicing Endonuclease'''<br />
 
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==Overview==
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==RNA Recognition and Cleavage by an Splicing Endonuclease==
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The RNA splicing endonuclease cleaves two phosphodiester bonds within, folded precursor RNAs during intron removal, producing the functional RNAs, required for protein synthesis. Here we describe at a resolution of 2.85, angstroms the structure of a splicing endonuclease from Archaeglobus, fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a, cleaved splice site. The endonuclease dimer cooperatively recognized a, flipped-out bulge base and stabilizes sharply bent bulge backbones that, are poised for an in-line RNA cleavage reaction. Cooperativity arises, because an arginine pair from one catalytic domain sandwiches a nucleobase, within the bulge cleaved by the other catalytic domain.
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<StructureSection load='2gjw' size='340' side='right'caption='[[2gjw]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gjw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjw OCA], [https://pdbe.org/2gjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gjw RCSB], [https://www.ebi.ac.uk/pdbsum/2gjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ENDA_ARCFU ENDA_ARCFU] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gjw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gjw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GJW OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16690865 16690865]
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: tRNA-intron endonuclease]]
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[[Category: Calvin K]]
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[[Category: Calvin, K.]]
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[[Category: Li H]]
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[[Category: Li, H.]]
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[[Category: Xue S]]
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[[Category: Xue, S.]]
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[[Category: bulge-helix-bulge rna-protein complex]]
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[[Category: splicing endonuclease af]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:14:47 2007''
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RNA Recognition and Cleavage by an Splicing Endonuclease

PDB ID 2gjw

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