2go2

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(New page: 200px<br /><applet load="2go2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2go2, resolution 1.87&Aring;" /> '''Crystal structure of...)
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[[Image:2go2.jpg|left|200px]]<br /><applet load="2go2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2go2, resolution 1.87&Aring;" />
 
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'''Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor'''<br />
 
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==Overview==
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==Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor==
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A Kunitz-type protease inhibitor (BbKI) found in Bauhinia bauhinioides, seeds has been overexpressed in Escherichia coli and crystallized at 293 K, using PEG 4000 as the precipitant. X-ray diffraction data have been, collected to 1.87 A resolution using an in-house X-ray generator. The, crystals of the recombinant protein (rBbKI) belong to the orthorhombic, space group P2(1)2(1)2(1), with unit-cell parameters a = 46.70, b = 64.14, c = 59.24 A. Calculation of the Matthews coefficient suggests the presence, of one monomer of rBbKI in the asymmetric unit, with a corresponding, solvent content of 51% (VM = 2.5 A3 Da(-1)). Iodinated crystals were, prepared and a derivative data set was also collected at 2.1 A resolution., Crystals soaked for a few seconds in a cryogenic solution containing 0.5 M, NaI were found to be reasonably isomorphous to the native crystals., Furthermore, the presence of iodide anions could be confirmed in the, NaI-derivatized crystal. Data sets from native and derivative crystals are, being evaluated for use in crystal structure determination by means of the, SIRAS (single isomorphous replacement with anomalous scattering) method.
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<StructureSection load='2go2' size='340' side='right'caption='[[2go2]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2go2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GO2 FirstGlance]. <br>
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2GO2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2go2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2go2 OCA], [https://pdbe.org/2go2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2go2 RCSB], [https://www.ebi.ac.uk/pdbsum/2go2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2go2 ProSAT]</span></td></tr>
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Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides., Navarro MV, Vierira DF, Nagem RA, de Araujo AP, Oliva ML, Garratt RC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):910-3. Epub 2005 Sep 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511193 16511193]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6VEQ7_BAUBA Q6VEQ7_BAUBA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/2go2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2go2 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bauhinia bauhinioides]]
[[Category: Bauhinia bauhinioides]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garratt, R.C.]]
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[[Category: Garratt RC]]
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[[Category: Navarro, M.V.A.S.]]
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[[Category: Navarro MVAS]]
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[[Category: EDO]]
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[[Category: beta-trefoil fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:04:27 2008''
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Current revision

Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor

PDB ID 2go2

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