2go2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:28, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2go2.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor==
-
The line below this paragraph, containing "STRUCTURE_2go2", creates the "Structure Box" on the page.
+
<StructureSection load='2go2' size='340' side='right'caption='[[2go2]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2go2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GO2 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-
{{STRUCTURE_2go2| PDB=2go2 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2go2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2go2 OCA], [https://pdbe.org/2go2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2go2 RCSB], [https://www.ebi.ac.uk/pdbsum/2go2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2go2 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/Q6VEQ7_BAUBA Q6VEQ7_BAUBA]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
A Kunitz-type protease inhibitor (BbKI) found in Bauhinia bauhinioides seeds has been overexpressed in Escherichia coli and crystallized at 293 K using PEG 4000 as the precipitant. X-ray diffraction data have been collected to 1.87 A resolution using an in-house X-ray generator. The crystals of the recombinant protein (rBbKI) belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 46.70, b = 64.14, c = 59.24 A. Calculation of the Matthews coefficient suggests the presence of one monomer of rBbKI in the asymmetric unit, with a corresponding solvent content of 51% (VM = 2.5 A3 Da(-1)). Iodinated crystals were prepared and a derivative data set was also collected at 2.1 A resolution. Crystals soaked for a few seconds in a cryogenic solution containing 0.5 M NaI were found to be reasonably isomorphous to the native crystals. Furthermore, the presence of iodide anions could be confirmed in the NaI-derivatized crystal. Data sets from native and derivative crystals are being evaluated for use in crystal structure determination by means of the SIRAS (single isomorphous replacement with anomalous scattering) method.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/2go2_consurf.spt"</scriptWhenChecked>
-
2GO2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides., Navarro MV, Vierira DF, Nagem RA, de Araujo AP, Oliva ML, Garratt RC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):910-3. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511193 16511193]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2go2 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Bauhinia bauhinioides]]
[[Category: Bauhinia bauhinioides]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Garratt, R C.]]
+
[[Category: Garratt RC]]
-
[[Category: Navarro, M V.A S.]]
+
[[Category: Navarro MVAS]]
-
[[Category: Beta-trefoil fold]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:19:44 2008''
+

Current revision

Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor

PDB ID 2go2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2go2"
Personal tools