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2goy

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[[Image:2goy.gif|left|200px]]
 
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{{Structure
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==Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS==
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|PDB= 2goy |SIZE=350|CAPTION= <scene name='initialview01'>2goy</scene>, resolution 2.700&Aring;
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<StructureSection load='2goy' size='340' side='right'caption='[[2goy]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=ADX:ADENOSINE-5&#39;-PHOSPHOSULFATE'>ADX</scene>
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<table><tr><td colspan='2'>[[2goy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GOY FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase_(thioredoxin) Adenylyl-sulfate reductase (thioredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.10 1.8.4.10]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE= cysH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2goy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2goy OCA], [https://pdbe.org/2goy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2goy RCSB], [https://www.ebi.ac.uk/pdbsum/2goy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2goy ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS'''
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== Function ==
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[https://www.uniprot.org/uniprot/CYSH_PSEAE CYSH_PSEAE] Reduction of activated sulfate into sulfite (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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APS reductase catalyzes the first committed step of reductive sulfate assimilation in pathogenic bacteria, including Mycobacterium tuberculosis, and is a promising target for drug development. We report the 2.7 A resolution crystal structure of Pseudomonas aeruginosa APS reductase in the thiosulfonate intermediate form of the catalytic cycle and with substrate bound. The structure, high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry, and quantitative kinetic analysis, establish that the two chemically discrete steps of the overall reaction take place at distinct sites on the enzyme, mediated via conformational flexibility of the C-terminal 18 residues. The results address the mechanism by which sulfonucleotide reductases protect the covalent but labile enzyme-intermediate before release of sulfite by the protein cofactor thioredoxin. P. aeruginosa APS reductase contains an [4Fe-4S] cluster that is essential for catalysis. The structure reveals an unusual mode of cluster coordination by tandem cysteine residues and suggests how this arrangement might facilitate conformational change and cluster interaction with the substrate. Assimilatory 3'-phosphoadenosine 5'-phosphosulfate (PAPS) reductases are evolutionarily related, homologous enzymes that catalyze the same overall reaction, but do so in the absence of an [Fe-S] cluster. The APS reductase structure reveals adaptive use of a phosphate-binding loop for recognition of the APS O3' hydroxyl group, or the PAPS 3'-phosphate group.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/2goy_consurf.spt"</scriptWhenChecked>
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2GOY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOY OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Substrate recognition, protein dynamics, and iron-sulfur cluster in Pseudomonas aeruginosa adenosine 5'-phosphosulfate reductase., Chartron J, Carroll KS, Shiau C, Gao H, Leary JA, Bertozzi CR, Stout CD, J Mol Biol. 2006 Nov 24;364(2):152-69. Epub 2006 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17010373 17010373]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2goy ConSurf].
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[[Category: Adenylyl-sulfate reductase (thioredoxin)]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Bertozzi CR]]
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[[Category: Bertozzi, C R.]]
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[[Category: Carroll KS]]
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[[Category: Carroll, K S.]]
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[[Category: Chartron J]]
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[[Category: Chartron, J.]]
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[[Category: Gao H]]
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[[Category: Gao, H.]]
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[[Category: Leary JA]]
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[[Category: Leary, J A.]]
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[[Category: Shiau C]]
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[[Category: Shiau, C.]]
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[[Category: Stout CD]]
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[[Category: Stout, C D.]]
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[[Category: ADX]]
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[[Category: SF4]]
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[[Category: adenosine 5'-phosphosulfate]]
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[[Category: iron sulfur cluster]]
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[[Category: nucleotide binding]]
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[[Category: thiosulfonate intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:11:39 2008''
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Current revision

Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS

PDB ID 2goy

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