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2hfn

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[[Image:2hfn.jpg|left|200px]]<br /><applet load="2hfn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hfn, resolution 1.80&Aring;" />
 
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'''Crystal Structures of the Synechocystis Photoreceptor Slr1694 Reveal Distinct Structural States Related to Signaling'''<br />
 
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==Overview==
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==Crystal Structures of the Synechocystis Photoreceptor Slr1694 Reveal Distinct Structural States Related to Signaling==
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Crystal structures of the Synechocystis BLUF phototaxis photoreceptor Slr1694 have been determined in two crystal forms, a monoclinic form at 1.8 A resolution and an orthorhombic form at 2.1 A resolution. In both forms, the photoreceptor is comprised of two pentamer rings stacked face to face. Twenty total subunits in the two asymmetric units of these crystal forms display three distinct tertiary structures that differ in the length of the fifth beta-strand and in the orientation of Trp91, a conserved Trp residue near the FMN chromophore. Fluorescence spectroscopic analysis on Slr1694 in solution is consistent with motion of Trp91 from a hydrophobic environment in the dark state to a more hydrophilic environment in the light-excited state. Mutational analysis indicates that movement of Trp91 is dependent on the occupancy of the hydrophobic Trp binding pocket with a nearby Met. These different tertiary structures may be associated with absorption changes in the blue region of the spectrum.
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<StructureSection load='2hfn' size='340' side='right'caption='[[2hfn]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2hfn]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HFN FirstGlance]. <br>
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2HFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hfn OCA], [https://pdbe.org/2hfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hfn RCSB], [https://www.ebi.ac.uk/pdbsum/2hfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hfn ProSAT]</span></td></tr>
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Crystal structures of the Synechocystis photoreceptor Slr1694 reveal distinct structural states related to signaling., Yuan H, Anderson S, Masuda S, Dragnea V, Moffat K, Bauer C, Biochemistry. 2006 Oct 24;45(42):12687-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17042486 17042486]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Synechocystis sp.]]
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[https://www.uniprot.org/uniprot/P74295_SYNY3 P74295_SYNY3]
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[[Category: Anderson, S.]]
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== Evolutionary Conservation ==
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[[Category: Bauer, C E.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Dragnea, V.]]
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Check<jmol>
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[[Category: Masuda, S.]]
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<jmolCheckbox>
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[[Category: Moffat, K.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hf/2hfn_consurf.spt"</scriptWhenChecked>
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[[Category: Yuan, H.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: FMN]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: beta sheet ferredoxin-like fold]]
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</jmolCheckbox>
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[[Category: flavin binding protein]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hfn ConSurf].
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[[Category: photoreceptor]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:41:22 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synechocystis sp]]
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[[Category: Anderson S]]
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[[Category: Bauer CE]]
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[[Category: Dragnea V]]
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[[Category: Masuda S]]
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[[Category: Moffat K]]
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[[Category: Yuan H]]

Current revision

Crystal Structures of the Synechocystis Photoreceptor Slr1694 Reveal Distinct Structural States Related to Signaling

PDB ID 2hfn

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