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2hit

From Proteopedia

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[[Image:2hit.gif|left|200px]]<br /><applet load="2hit" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hit, resolution 2.75&Aring;" />
 
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'''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine'''<br />
 
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==Overview==
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==Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine==
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This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.
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<StructureSection load='2hit' size='340' side='right'caption='[[2hit]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2hit]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIT FirstGlance]. <br>
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2HIT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=BCL:'>BCL</scene>, <scene name='pdbligand=BPH:'>BPH</scene>, <scene name='pdbligand=U10:'>U10</scene>, <scene name='pdbligand=CDL:'>CDL</scene>, <scene name='pdbligand=PEV:'>PEV</scene>, <scene name='pdbligand=PEW:'>PEW</scene>, <scene name='pdbligand=LDA:'>LDA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PEV:(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+STEARATE'>PEV</scene>, <scene name='pdbligand=PEW:(1R)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(9S,10S)-9,10-DIBROMOOCTADECANOATE'>PEW</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hit OCA], [https://pdbe.org/2hit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hit RCSB], [https://www.ebi.ac.uk/pdbsum/2hit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hit ProSAT]</span></td></tr>
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Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17315985 17315985]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEL_CERSP RCEL_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Cogdell, R J.]]
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== Evolutionary Conservation ==
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[[Category: Gardiner, A T.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Isaacs, N W.]]
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Check<jmol>
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[[Category: Roszak, A W.]]
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<jmolCheckbox>
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[[Category: BCL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/2hit_consurf.spt"</scriptWhenChecked>
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[[Category: BPH]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: CDL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: CL]]
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</jmolCheckbox>
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[[Category: FE]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hit ConSurf].
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[[Category: GOL]]
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<div style="clear:both"></div>
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[[Category: K]]
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__TOC__
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[[Category: LDA]]
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</StructureSection>
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[[Category: PEV]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: PEW]]
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[[Category: Large Structures]]
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[[Category: PO4]]
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[[Category: Cogdell RJ]]
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[[Category: U10]]
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[[Category: Gardiner AT]]
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[[Category: brominated lipids]]
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[[Category: Isaacs NW]]
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[[Category: lipid binding sites]]
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[[Category: Roszak AW]]
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[[Category: membrane protein]]
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[[Category: photosynthesis]]
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[[Category: photosynthetic reaction center]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:42:22 2008''
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Current revision

Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine

PDB ID 2hit

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