2hqw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2hqw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hqw, resolution 1.90&Aring;" /> '''Crystal Structure of...)
Current revision (09:34, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2hqw.jpg|left|200px]]<br /><applet load="2hqw" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2hqw, resolution 1.90&Aring;" />
 
-
'''Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide'''<br />
 
-
==Overview==
+
==Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide==
-
Calmodulin (CaM) regulates tetrameric N-methyl-D-aspartate receptors, (NMDARs) by binding tightly to the C0 and C1 regions of its NR1 subunit. A, crystal structure (2HQW; 1.96 A) of calcium-saturated CaM bound to NR1C1, (peptide spanning 875-898) showed that NR1 S890, whose phosphorylation, regulates membrane localization, was solvent protected, whereas the, endoplasmic reticulum retention motif was solvent exposed. NR1 F880 filled, the CaM C-domain pocket, whereas T886 was closest to the N-domain pocket., This 1-7 pattern was most similar to that in the CaM-MARCKS complex., Comparison of CaM-ligand wrap-around conformations identified a core, tetrad of CaM C-domain residues (FLMM(C)) that contacted all ligands, consistently. An identical tetrad of N-domain residues (FLMM(N)) made, variable sets of contacts with ligands. This CaM-NR1C1 structure provides, a foundation for designing mutants to test the role of CaM in NR1, trafficking as well as insights into how the homologous CaM domains have, different roles in molecular recognition.
+
<StructureSection load='2hqw' size='340' side='right'caption='[[2hqw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2hqw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQW FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqw OCA], [https://pdbe.org/2hqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqw RCSB], [https://www.ebi.ac.uk/pdbsum/2hqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqw ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CALM1_RAT CALM1_RAT] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB:P62158]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqw ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2HQW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQW OCA].
+
*[[Calmodulin 3D structures|Calmodulin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The NMDA Receptor NR1 C1 Region Bound to Calmodulin: Structural Insights into Functional Differences between Homologous Domains., Ataman ZA, Gakhar L, Sorensen BR, Hell JW, Shea MA, Structure. 2007 Dec;15(12):1603-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18073110 18073110]
+
[[Category: Homo sapiens]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Akyol, Z.]]
+
[[Category: Akyol Z]]
-
[[Category: Gakhar, L.]]
+
[[Category: Gakhar L]]
-
[[Category: Hell, J.H.]]
+
[[Category: Hell JH]]
-
[[Category: Shea, M.A.]]
+
[[Category: Shea MA]]
-
[[Category: Sorensen, B.R.]]
+
[[Category: Sorensen BR]]
-
[[Category: CA]]
+
-
[[Category: alternative splicing]]
+
-
[[Category: c1 casette]]
+
-
[[Category: calcium channel]]
+
-
[[Category: calmodulin]]
+
-
[[Category: central nervous system]]
+
-
[[Category: ef hand motif]]
+
-
[[Category: er retention signal]]
+
-
[[Category: globular complex]]
+
-
[[Category: glutamate]]
+
-
[[Category: ion channel]]
+
-
[[Category: metal binding protein]]
+
-
[[Category: n-methyl-d-aspartate receptor]]
+
-
[[Category: neuronal channel]]
+
-
[[Category: nr1]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:22:10 2008''
+

Current revision

Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide

PDB ID 2hqw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hqw"
Personal tools