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3nht

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==Crystal structure of the AnSt-D7L1-U46619 complex==
==Crystal structure of the AnSt-D7L1-U46619 complex==
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<StructureSection load='3nht' size='340' side='right' caption='[[3nht]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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<StructureSection load='3nht' size='340' side='right'caption='[[3nht]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nht]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anost Anost]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NHT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nht]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_stephensi Anopheles stephensi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NHT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U46:(5E)-7-{6-[(1E)-3-HYDROXYOCT-1-ENYL]-2-OXABICYCLO[2.2.1]HEPT-5-YL}HEPT-5-ENOIC+ACID'>U46</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nhi|3nhi]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U46:(5E)-7-{6-[(1E)-3-HYDROXYOCT-1-ENYL]-2-OXABICYCLO[2.2.1]HEPT-5-YL}HEPT-5-ENOIC+ACID'>U46</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nht OCA], [http://pdbe.org/3nht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nht RCSB], [http://www.ebi.ac.uk/pdbsum/3nht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nht ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nht OCA], [https://pdbe.org/3nht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nht RCSB], [https://www.ebi.ac.uk/pdbsum/3nht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nht ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q95NY5_ANOST Q95NY5_ANOST]
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The highly expressed D7 protein family of mosquito saliva has previously been shown to act as an anti-inflammatory mediator by binding host biogenic amines and cysteinyl leukotrienes (CysLTs). In this study we demonstrate that AnSt-D7L1, a two-domain member of this group from Anopheles stephensi, retains the CysLT binding function seen in the homolog AeD7 from Aedes aegypti but has lost the ability to bind biogenic amines. Unlike any previously characterized members of the D7 family, AnSt-D7L1 has acquired the important function of binding thromboxane A(2) (TXA(2)) and its analogs with high affinity. When administered to tissue preparations, AnSt-D7L1 abrogated Leukotriene C(4) (LTC(4))-induced contraction of guinea pig ileum and contraction of rat aorta by the TXA(2) analog U46619. The protein also inhibited platelet aggregation induced by both collagen and U46619 when administered to stirred platelets. The crystal structure of AnSt-D7L1 contains two OBP-like domains and has a structure similar to AeD7. In AnSt-D7L1, the binding pocket of the C-terminal domain has been rearranged relative to AeD7, making the protein unable to bind biogenic amines. Structures of the ligand complexes show that CysLTs and TXA(2) analogs both bind in the same hydrophobic pocket of the N-terminal domain. The TXA(2) analog U46619 is stabilized by hydrogen bonding interactions of the omega-5 hydroxyl group with the phenolic hydroxyl group of Tyr 52. LTC(4) and occupies a very similar position to LTE(4) in the previously determined structure of its complex with AeD7. As yet, it is not known what, if any, new function has been acquired by the rearranged C-terminal domain. This article presents, to our knowledge, the first structural characterization of a protein from mosquito saliva that inhibits collagen mediated platelet activation.
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The function and three-dimensional structure of a thromboxane A2/cysteinyl leukotriene-binding protein from the saliva of a mosquito vector of the malaria parasite.,Alvarenga PH, Francischetti IM, Calvo E, Sa-Nunes A, Ribeiro JM, Andersen JF PLoS Biol. 2010 Nov 30;8(11):e1000547. PMID:21152418<ref>PMID:21152418</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nht" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Anost]]
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[[Category: Anopheles stephensi]]
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[[Category: Alvarenga, P H]]
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[[Category: Large Structures]]
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[[Category: Andersen, J F]]
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[[Category: Alvarenga PH]]
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[[Category: Calvo, E]]
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[[Category: Andersen JF]]
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[[Category: Francischetti, I M]]
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[[Category: Calvo E]]
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[[Category: Ribeiro, J M]]
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[[Category: Francischetti IM]]
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[[Category: Sa-Nunes, A]]
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[[Category: Ribeiro JM]]
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[[Category: All-helical]]
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[[Category: Sa-Nunes A]]
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[[Category: D7]]
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[[Category: Ligand-binding protein]]
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[[Category: Odorant-binding protein]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of the AnSt-D7L1-U46619 complex

PDB ID 3nht

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