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3o5z

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Crystal structure of the SH3 domain from p85beta subunit of phosphoinositide 3-kinase (PI3K)

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 ==Crystal structure of the SH3 domain from p85beta subunit of phosphoinositide 3-kinase (PI3K)==
-<StructureSection load='3o5z' size='340' side='right' caption='[[3o5z]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
+<StructureSection load='3o5z' size='340' side='right'caption='[[3o5z]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O5Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3o5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O5Z FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIK3R2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o5z RCSB], [http://www.ebi.ac.uk/pdbsum/3o5z PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o5z OCA], [https://pdbe.org/3o5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o5z RCSB], [https://www.ebi.ac.uk/pdbsum/3o5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o5z ProSAT]</span></td></tr>
-<table>
+</table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/P85B_HUMAN P85B_HUMAN] Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
-Src-homology 3 (SH3) domains are involved in extensive protein-protein interactions and constitute key elements of intracellular signal transduction. Three-dimensional structures have been reported for SH3 domains of various proteins, including the 85 kDa regulatory subunit (p85) of phosphoinositide 3-kinase. However, all of the latter structures are of p85 isoform alpha and no crystal structure of the SH3 domain of the equally important isoform beta has been reported to date. In this structural communication, the recombinant production, crystallization and X-ray structure determination at 2.0 A resolution of the SH3 domain of human p85beta is described. The structure reveals a compact beta-barrel fold very similar to that of p85alpha. However, binding studies with two classes of proline-rich ligand peptides demonstrate that the ligand-binding specificity differs slightly between the SH3 domains of human p85beta and p85alpha, despite their high structural similarity.
-X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85beta subunit.,Chen S, Xiao Y, Ponnusamy R, Tan J, Lei J, Hilgenfeld R Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1328-33., Epub 2011 Oct 25. PMID:22102226<ref>PMID:22102226</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 ==See Also==
-*[[Phosphoinositide 3-Kinases|Phosphoinositide 3-Kinases]]
+*[[Phosphoinositide 3-kinase 3D structures|Phosphoinositide 3-kinase 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Chen, S.]]
+[[Category: Large Structures]]
-[[Category: Hilgenfeld, R.]]
+[[Category: Chen S]]
-[[Category: Lei, J.]]
+[[Category: Hilgenfeld R]]
-[[Category: Ponnusamy, R.]]
+[[Category: Lei J]]
-[[Category: Tan, J.]]
+[[Category: Ponnusamy R]]
-[[Category: Xiao, Y.]]
+[[Category: Tan J]]
-[[Category: Protein binding]]
+[[Category: Xiao Y]]
-[[Category: Src homology 3 domain]]

3o5z

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Crystal structure of the SH3 domain from p85beta subunit of phosphoinositide 3-kinase (PI3K)

Structural highlights

Function

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