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2hyd

From Proteopedia

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(New page: 200px<br /><applet load="2hyd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hyd, resolution 3.0&Aring;" /> '''Multidrug ABC transpo...)
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[[Image:2hyd.gif|left|200px]]<br /><applet load="2hyd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hyd, resolution 3.0&Aring;" />
 
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'''Multidrug ABC transporter SAV1866'''<br />
 
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==Overview==
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==Multidrug ABC transporter SAV1866==
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Multidrug transporters of the ABC family facilitate the export of diverse, cytotoxic drugs across cell membranes. This is clinically relevant, as, tumour cells may become resistant to agents used in chemotherapy. To, understand the molecular basis of this process, we have determined the 3.0, A crystal structure of a bacterial ABC transporter (Sav1866) from, Staphylococcus aureus. The homodimeric protein consists of 12, transmembrane helices in an arrangement that is consistent with, cross-linking studies and electron microscopic imaging of the human, multidrug resistance protein MDR1, but critically different from that, reported for the bacterial lipid flippase MsbA. The observed, outward-facing conformation reflects the ATP-bound state, with the two, nucleotide-binding domains in close contact and the two transmembrane, domains forming a central cavity--presumably the drug translocation, pathway--that is shielded from the inner leaflet of the lipid bilayer and, from the cytoplasm, but exposed to the outer leaflet and the extracellular, space.
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<StructureSection load='2hyd' size='340' side='right'caption='[[2hyd]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hyd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HYD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hyd OCA], [https://pdbe.org/2hyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hyd RCSB], [https://www.ebi.ac.uk/pdbsum/2hyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hyd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Y1866_STAAM Y1866_STAAM] May be involved in multidrug export. Transmembrane domains (TMD) form a pore in the cell membrane and the ATP-binding domain (NBD) is responsible for energy generation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hyd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hyd ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NA and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HYD OCA].
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of a bacterial multidrug ABC transporter., Dawson RJ, Locher KP, Nature. 2006 Sep 14;443(7108):180-5. Epub 2006 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16943773 16943773]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Dawson, R.J.P.]]
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[[Category: Dawson RJP]]
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[[Category: Locher, K.P.]]
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[[Category: Locher KP]]
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[[Category: ADP]]
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[[Category: NA]]
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[[Category: transport protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:03:23 2007''
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Current revision

Multidrug ABC transporter SAV1866

PDB ID 2hyd

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