2i7t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2i7t" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i7t, resolution 2.10&Aring;" /> '''Structure of human ...)
Current revision (09:02, 21 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2i7t.gif|left|200px]]<br />
 
-
<applet load="2i7t" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2i7t, resolution 2.10&Aring;" />
 
-
'''Structure of human CPSF-73'''<br />
 
-
==Overview==
+
==Structure of human CPSF-73==
-
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive, maturational processing, including cleavage and polyadenylation at the, 3'-end. Despite the characterization of many proteins that are required, for the cleavage reaction, the identity of the endonuclease is not known., Recent analyses indicated that the 73-kDa subunit of cleavage and, polyadenylation specificity factor (CPSF-73) might be the endonuclease for, this and related reactions, although no direct data confirmed this. Here, we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate, group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at, 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a, metallo-beta-lactamase domain and a novel beta-CASP (named for, metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site, of CPSF-73, with two zinc ions, is located at the interface of the two, domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this, activity. Our studies provide the first direct experimental evidence that, CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
+
<StructureSection load='2i7t' size='340' side='right'caption='[[2i7t]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2i7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I7T FirstGlance]. <br>
-
2I7T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2I7T OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7t OCA], [https://pdbe.org/2i7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i7t RCSB], [https://www.ebi.ac.uk/pdbsum/2i7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i7t ProSAT]</span></td></tr>
-
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17128255 17128255]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CPSF3_HUMAN CPSF3_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.<ref>PMID:14749727</ref> <ref>PMID:15037765</ref> <ref>PMID:18688255</ref> <ref>PMID:17128255</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/2i7t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i7t ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Mandel, C.R.]]
+
[[Category: Mandel CR]]
-
[[Category: Tong, L.]]
+
[[Category: Tong L]]
-
[[Category: Zhang, H.]]
+
[[Category: Zhang H]]
-
[[Category: SO4]]
+
-
[[Category: ZN]]
+
-
[[Category: artemis]]
+
-
[[Category: double-strand break repair]]
+
-
[[Category: metallo-b-lactamase]]
+
-
[[Category: polyadenylation]]
+
-
[[Category: pre-mrna processing]]
+
-
[[Category: v(d)j recombination]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:42:17 2007''
+

Current revision

Structure of human CPSF-73

PDB ID 2i7t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i7t"
Personal tools