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2i8u

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(New page: 200px<br /><applet load="2i8u" size="350" color="white" frame="true" align="right" spinBox="true" caption="2i8u, resolution 1.400&Aring;" /> '''GDP-mannose mannosy...)
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[[Image:2i8u.jpg|left|200px]]<br /><applet load="2i8u" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2i8u, resolution 1.400&Aring;" />
 
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'''GDP-mannose mannosyl hydrolase-calcium-GDP product complex'''<br />
 
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==Overview==
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==GDP-mannose mannosyl hydrolase-calcium-GDP product complex==
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Diversity in the polysaccharide component of lipopolysaccharide (LPS), contributes to the persistence and pathogenesis of Gram-negative bacteria., The Nudix hydrolase GDP-mannose mannosyl hydrolase (Gmm) contributes to, this diversity by regulating the concentration of mannose in LPS, biosynthetic pathways. Here, we present seven high-resolution crystal, structures of Gmm from the enteropathogenic E. coli strain O128: the, structure of the apo enzyme, the cocrystal structure of Gmm bound to the, product Mg2+-GDP, two cocrystal structures of precatalytic and turnover, complexes of Gmm-Ca2+-GDP-alpha-d-mannose, and three cocrystal structures, of an inactive mutant (His-124 --&gt; Leu) Gmm bound to substrates, GDP-alpha-d-mannose, GDP-alpha-d-glucose, and GDP-beta-l-fucose. These, crystal structures help explain the molecular basis for substrate, specificity and promiscuity and provide a structural framework for, reconciling previously determined kinetic parameters. Unexpectedly, these, structures reveal concerted changes in the enzyme structure that result in, the formation of a catalytically competent active site only in the, presence of the substrate/product. These structural views of the enzyme, may provide a rationale for the design of inhibitors that target the, biosynthesis of LPS by pathogenic bacteria.
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<StructureSection load='2i8u' size='340' side='right'caption='[[2i8u]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2i8u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I8U FirstGlance]. <br>
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2I8U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i8u OCA], [https://pdbe.org/2i8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i8u RCSB], [https://www.ebi.ac.uk/pdbsum/2i8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i8u ProSAT]</span></td></tr>
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Molecular basis for substrate selectivity and specificity by an LPS biosynthetic enzyme., Zou Y, Li C, Brunzelle JS, Nair SK, Biochemistry. 2007 Apr 10;46(14):4294-304. Epub 2007 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17371001 17371001]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6XQ58_ECOLX Q6XQ58_ECOLX] Hydrolyzes GDP-mannose.[HAMAP-Rule:MF_00941]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/2i8u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i8u ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brunzelle, J.S.]]
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[[Category: Brunzelle JS]]
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[[Category: Li, C.]]
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[[Category: Li C]]
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[[Category: Nair, S.K.]]
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[[Category: Nair SK]]
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[[Category: Zou, Y.]]
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[[Category: Zou Y]]
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[[Category: CA]]
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[[Category: GDP]]
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[[Category: lipopolysaccharide]]
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[[Category: nudix enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:49:11 2008''
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Current revision

GDP-mannose mannosyl hydrolase-calcium-GDP product complex

PDB ID 2i8u

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