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| - | [[Image:2il9.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms== |
| - | |PDB= 2il9 |SIZE=350|CAPTION= <scene name='initialview01'>2il9</scene>, resolution 3.100Å
| + | <StructureSection load='2il9' size='340' side='right'caption='[[2il9]], [[Resolution|resolution]] 3.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2il9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plautia_stali_intestine_virus Plautia stali intestine virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IL9 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2il9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2il9 OCA], [https://pdbe.org/2il9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2il9 RCSB], [https://www.ebi.ac.uk/pdbsum/2il9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2il9 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2il9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2il9 OCA], [http://www.ebi.ac.uk/pdbsum/2il9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2il9 RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Plautia stali intestine virus]] |
| - | '''Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms'''
| + | [[Category: Costantino DA]] |
| - | | + | [[Category: Kieft JS]] |
| - | | + | [[Category: Pfingsten JS]] |
| - | ==Overview==
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| - | Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.
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| - | ==About this Structure==
| + | |
| - | 2IL9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IL9 OCA].
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| - | ==Reference==
| + | |
| - | Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17124290 17124290]
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| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Costantino, D A.]]
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| - | [[Category: Kieft, J S.]] | + | |
| - | [[Category: Pfingsten, J S.]] | + | |
| - | [[Category: double-nested pseudoknot]] | + | |
| - | [[Category: ire]] | + | |
| - | [[Category: psiv]] | + | |
| - | [[Category: ribosomal binding domain]]
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| - | [[Category: rna]]
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| - | [[Category: under wound helix]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:45:43 2008''
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