This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2p6r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2p6r" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p6r, resolution 3.000&Aring;" /> '''Crystal structure o...)
Current revision (09:08, 21 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2p6r.gif|left|200px]]<br /><applet load="2p6r" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2p6r, resolution 3.000&Aring;" />
 
-
'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA'''<br />
 
-
==Overview==
+
==Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA==
-
To reveal the mechanism of processive strand separation by superfamily-2, (SF2) 3'--&gt;5' helicases, we determined apo and DNA-bound crystal, structures of archaeal Hel308, a helicase that unwinds lagging strands and, is related to human DNA polymerase theta. Our structure captures the, duplex-unwinding reaction, shows that initial strand separation does not, require ATP and identifies a prominent beta-hairpin loop as the unwinding, element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay, factors support the idea that this duplex-unwinding mechanism is, applicable to a broad subset of SF2 helicases. Comparison with ATP-bound, SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair, by ratchet-like transport of the 3' product strand. Our results provide a, first structural framework for strand separation by processive SF2 3'--&gt;5', helicases and reveal important mechanistic differences from SF1 helicases.
+
<StructureSection load='2p6r' size='340' side='right'caption='[[2p6r]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2p6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6R FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [https://pdbe.org/2p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB], [https://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6r ProSAT]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6r_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6r ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2P6R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA].
+
*[[Helicase 3D structures|Helicase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17558417 17558417]
+
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Buettner, K.]]
+
[[Category: Buettner K]]
-
[[Category: Hopfner, K.P.]]
+
[[Category: Hopfner KP]]
-
[[Category: Nehring, S.]]
+
[[Category: Nehring S]]
-
[[Category: archaeal helicase]]
+
-
[[Category: dna repair]]
+
-
[[Category: protein-dna complex]]
+
-
[[Category: sf2 helicase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:43:10 2008''
+

Current revision

Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA

PDB ID 2p6r

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p6r"
Personal tools