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2p6u

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[[Image:2p6u.jpg|left|200px]]<br /><applet load="2p6u" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p6u, resolution 3.14&Aring;" />
 
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'''Apo structure of the Hel308 superfamily 2 helicase'''<br />
 
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==Overview==
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==Apo structure of the Hel308 superfamily 2 helicase==
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To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'--&gt;5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'--&gt;5' helicases and reveal important mechanistic differences from SF1 helicases.
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<StructureSection load='2p6u' size='340' side='right'caption='[[2p6u]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p6u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.14&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6u OCA], [https://pdbe.org/2p6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6u RCSB], [https://www.ebi.ac.uk/pdbsum/2p6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6u ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6u ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2P6U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6U OCA].
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*[[Helicase 3D structures|Helicase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17558417 17558417]
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Buettner, K.]]
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[[Category: Buettner K]]
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[[Category: Hopfner, K P.]]
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[[Category: Hopfner KP]]
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[[Category: Nehring, S.]]
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[[Category: Nehring S]]
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[[Category: PO4]]
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[[Category: archaeal helicase]]
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[[Category: dna repair]]
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[[Category: sf2 helicase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:29 2008''
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Current revision

Apo structure of the Hel308 superfamily 2 helicase

PDB ID 2p6u

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