2p6w

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[[Image:2p6w.jpg|left|200px]]<br /><applet load="2p6w" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p6w, resolution 1.600&Aring;" />
 
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'''Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''<br />
 
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==Overview==
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==Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1==
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Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
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<StructureSection load='2p6w' size='340' side='right'caption='[[2p6w]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2p6w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6W FirstGlance]. <br>
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2P6W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=FLC:'>FLC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6w OCA], [https://pdbe.org/2p6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6w RCSB], [https://www.ebi.ac.uk/pdbsum/2p6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6w ProSAT]</span></td></tr>
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Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17850743 17850743]
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</table>
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[[Category: Paramecium bursaria chlorella virus 1]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1]
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[[Category: Etten, J L.Van.]]
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__TOC__
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[[Category: Rossmann, M G.]]
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</StructureSection>
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[[Category: Ye, X.]]
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[[Category: Large Structures]]
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[[Category: Zhang, Y.]]
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[[Category: Paramecium bursaria Chlorella virus 1]]
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[[Category: FLC]]
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[[Category: Rossmann MG]]
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[[Category: MN]]
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[[Category: Van Etten JL]]
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[[Category: crystal structure]]
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[[Category: Ye X]]
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[[Category: glycosyltransferase]]
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[[Category: Zhang Y]]
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[[Category: pbcv]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:28 2008''
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Current revision

Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1

PDB ID 2p6w

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