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2p9i

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[[Image:2p9i.jpg|left|200px]]<br /><applet load="2p9i" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p9i, resolution 2.460&Aring;" />
 
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'''Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde'''<br />
 
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==Overview==
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==Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde==
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ATP is required for nucleation of actin filament branches by Arp2/3, complex, but the influence of ATP binding and hydrolysis are poorly, understood. We determined crystal structures of bovine Arp2/3 complex, cocrystallized with various bound adenine nucleotides and cations., Nucleotide binding favors closure of the nucleotide-binding cleft of Arp3, but no large-scale conformational changes in the complex. Thus, ATP, binding does not directly activate Arp2/3 complex but is part of a network, of interactions that contribute to nucleation. We compared, nucleotide-induced conformational changes of residues lining the cleft in, Arp3 and actin structures to construct a movie depicting the proposed, ATPase cycle for the actin family. Chemical crosslinking stabilized, subdomain 1 of Arp2, revealing new electron density for 69 residues in, this subdomain. Steric clashes with Arp3 appear to be responsible for, intrinsic disorder of subdomains 1 and 2 of Arp2 in inactive Arp2/3, complex.
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<StructureSection load='2p9i' size='340' side='right'caption='[[2p9i]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p9i]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P9I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.46&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p9i OCA], [https://pdbe.org/2p9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p9i RCSB], [https://www.ebi.ac.uk/pdbsum/2p9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p9i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/2p9i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p9i ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2P9I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P9I OCA].
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*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Insights into the Influence of Nucleotides on Actin Family Proteins from Seven Structures of Arp2/3 Complex., Nolen BJ, Pollard TD, Mol Cell. 2007 May 11;26(3):449-457. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17499050 17499050]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Nolen, B.J.]]
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[[Category: Nolen BJ]]
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[[Category: Pollard, T.D.]]
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[[Category: Pollard TD]]
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[[Category: ADP]]
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[[Category: CA]]
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[[Category: actin]]
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[[Category: complex]]
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[[Category: wd repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:07:21 2008''
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Current revision

Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde

PDB ID 2p9i

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